Journal article
Genetically targeted radiotherapy of head and neck squamous cell carcinoma using the sodium-iodide symporter (NIS)
Head & neck, Vol.26(3), pp.265-271
2004
DOI: 10.1002/hed.10369
PMID: 14999802
Abstract
Background.
Gene therapy that uses delivery of the sodium‐iodide symporter (NIS) gene followed by radioiodide administration has been proposed as a novel form of radiotherapy for nonthyroidal cancers.
Methods.
In vitro [125I] iodide accumulation and efflux from cells was determined after treatment with an NIS‐expressing adenovirus (Ad‐NIS). A clonogenic survival assay and tumor growth experiment that used athymic mice were used to demonstrate the in vitro and in vivo cytotoxicity of Ad‐NIS treatment and [131I] iodide delivery.
Results.
Head and neck squamous cell carcinoma (HNSCC) cell lines treated with Ad‐NIS exhibit significant amounts of radioiodide accumulation and retention. In vitro HNSCC cell survival was significantly diminished after NIS gene delivery followed by administration of [131I] iodide. Moreover, NIS gene transfer/[131I] iodide administration dramatically attenuated HNSCC tumor formation in athymic mice.
Conclusions.
Our data demonstrate the feasibility of genetically targeted radiotherapy by use of the NIS gene as a possible therapeutic intervention in head and neck cancer.
Details
- Title: Subtitle
- Genetically targeted radiotherapy of head and neck squamous cell carcinoma using the sodium-iodide symporter (NIS)
- Creators
- Andrew W GAUT - Department of Otolaryngology-Head and Neck Surgery, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United StatesGang Niu - Free Radical and Radiation Biology Program, Department of Radiation Oncology, B 180 Med Labs, Iowa City, Iowa 52242, United StatesKimberly J KRAGER - Free Radical and Radiation Biology Program, Department of Radiation Oncology, B 180 Med Labs, Iowa City, Iowa 52242, United StatesMichael M GRAHAM - Division of Nuclear Medicine, Department of Radiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United StatesDouglas K TRASK - Department of Otolaryngology-Head and Neck Surgery, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United StatesFrederick E DOMANN - Free Radical and Radiation Biology Program, Department of Radiation Oncology, B 180 Med Labs, Iowa City, Iowa 52242, United States
- Resource Type
- Journal article
- Publication Details
- Head & neck, Vol.26(3), pp.265-271
- Publisher
- John Wiley & Sons; New York, NY
- DOI
- 10.1002/hed.10369
- PMID
- 14999802
- ISSN
- 1043-3074
- eISSN
- 1097-0347
- Language
- English
- Date published
- 2004
- Academic Unit
- Radiology; Pathology; Surgery; Radiation Oncology
- Record Identifier
- 9984047878102771
Metrics
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