Genome-wide analysis of DNA methylation in human amnion

Jinsil Kim; Mitchell M Pitlick; Paul J Christine; Amanda R Schaefer; Cesar Saleme; Belén Comas; Viviana Cosentino; Enrique Gadow; Jeffrey C Murray

doi:10.1155/2013/678156

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Genome-wide analysis of DNA methylation in human amnion

Journal article

Open access

Genome-wide analysis of DNA methylation in human amnion

Jinsil Kim, Mitchell M Pitlick, Paul J Christine, Amanda R Schaefer, Cesar Saleme, Belén Comas, Viviana Cosentino, Enrique Gadow and Jeffrey C Murray

TheScientificWorld, Vol.2013, 678156

2013

DOI: 10.1155/2013/678156

PMCID: PMC3590748

PMID: 23533356

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Abstract

The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies.

Pregnancy

Promoter Regions, Genetic

Premature Birth - genetics

Labor, Obstetric - physiology

Oligonucleotide Array Sequence Analysis

Receptors, Oxytocin - genetics

Amnion - cytology

Epigenesis, Genetic

Humans

Genetic Association Studies - methods

Gene Expression Profiling

Genetic Loci

Gestational Age

DNA Methylation

Premature Birth - physiopathology

Placenta - cytology

CpG Islands

Cation Transport Proteins - genetics

Female

Labor, Obstetric - genetics

Principal Component Analysis

Infant, Newborn

Details

Title: Subtitle: Genome-wide analysis of DNA methylation in human amnion
Creators: Jinsil Kim - Department of Anatomy and Cell Biology, University of Iowa, 500 Newton Road, 2182 ML, Iowa City, IA 52242, USA
Mitchell M Pitlick
Paul J Christine
Amanda R Schaefer
Cesar Saleme
Belén Comas
Viviana Cosentino
Enrique Gadow
Jeffrey C Murray
Resource Type: Journal article
Publication Details: TheScientificWorld, Vol.2013, 678156
DOI: 10.1155/2013/678156
PMID: 23533356
PMCID: PMC3590748
NLM abbreviation: ScientificWorldJournal
ISSN: 1537-744X
eISSN: 1537-744X
Publisher: United States
Grant note: R01 HD057192 / NICHD NIH HHS P30 ES005605 / NIEHS NIH HHS R01 HD052953 / NICHD NIH HHS HD57192 / NICHD NIH HHS
Language: English
Date published: 2013
Academic Unit: Anatomy and Cell Biology; Stead Family Department of Pediatrics; Epidemiology; Pediatric Dentistry; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984025349002771

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