Journal article
Genome-wide miRNA profiling of mantle cell lymphoma reveals a distinct subgroup with poor prognosis
Blood, Vol.119(21), pp.4939-4948
05/24/2012
DOI: 10.1182/blood-2011-07-370122
PMCID: PMC3367895
PMID: 22490335
Abstract
miRNA deregulation has been implicated in the pathogenesis of mantle cell lymphoma (MCL). Using a high-throughput quantitative real-time PCR platform, we performed miRNA profiling on cyclin D1–positive MCL (n = 30) and cyclin D1–negative MCL (n = 7) and compared them with small lymphocytic leukemia/lymphoma (n = 12), aggressive B-cell lymphomas (n = 138), normal B-cell subsets, and stromal cells. We identified a 19-miRNA classifier that included 6 up-regulated miRNAs and 13 down regulated miRNA that was able to distinguish MCL from other aggressive lymphomas. Some of the up-regulated miRNAs are highly expressed in naive B cells. This miRNA classifier showed consistent results in formalin-fixed paraffin-embedded tissues and was able to distinguish cyclin D1–negative MCL from other lymphomas. A 26-miRNA classifier could distinguish MCL from small lymphocytic leukemia/lymphoma, dominated by 23 up-regulated miRNAs in MCL. Unsupervised hierarchical clustering of MCL patients demonstrated a cluster characterized by high expression of miRNAs from the polycistronic miR17-92 cluster and its paralogs, miR-106a-363 and miR-106b-25, and associated with high proliferation gene signature. The other clusters showed enrichment of stroma-associated miRNAs, and also had higher expression of stroma-associated genes. Our clinical outcome analysis in the present study suggested that miRNAs can serve as prognosticators.
Details
- Title: Subtitle
- Genome-wide miRNA profiling of mantle cell lymphoma reveals a distinct subgroup with poor prognosis
- Creators
- Javeed Iqbal - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NEYulei Shen - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NEYanyan Liu - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NEKai Fu - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NEElaine S Jaffe - Laboratory of Pathology, Center for Cancer Research, National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MDCuiling Liu - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NEZhongfeng Liu - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NECynthia M Lachel - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NEKaren Deffenbacher - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NETimothy C Greiner - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NEJulie M Vose - Department of Hematology/Oncology, University of Nebraska Medical Center, Omaha, NESharathkumar Bhagavathi - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NELouis M Staudt - Metabolism Branch, Center for Cancer Research, NCI, NIH, Bethesda, MDLisa Rimsza - Department of Pathology, University of Arizona, Tucson, AZAndreas Rosenwald - Department of Pathology, University of Würzburg, Würzburg, GermanyGerman Ott - Department of Clinical Pathology, Dr Margarete Fischer Bosch Institute of Clinical Pharmacology, Robert Bosch Hospital, Stuttgart, GermanyJan Delabie - Department of Pathology, The Norwegian Radium Hospital, University of Oslo, Oslo, NorwayElias Campo - Hospital Clinic, University of Barcelona, Barcelona, SpainRita M Braziel - Department of Clinical Pathology, Oregon Health and Science University, Portland, ORJames R Cook - Department of Molecular Pathology and Laboratory Medicine, Cleveland Clinic, Cleveland, OH; andRaymond R Tubbs - Department of Molecular Pathology and Laboratory Medicine, Cleveland Clinic, Cleveland, OH; andRandy D Gascoyne - Center for Lymphoid Cancer, British Columbia Cancer Agency, Vancouver, BCJames O Armitage - Department of Hematology/Oncology, University of Nebraska Medical Center, Omaha, NEDennis D Weisenburger - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NETimothy W McKeithan - Department of Hematology/Oncology, University of Nebraska Medical Center, Omaha, NEWing C Chan - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.119(21), pp.4939-4948
- Publisher
- American Society of Hematology; Washington, DC
- DOI
- 10.1182/blood-2011-07-370122
- PMID
- 22490335
- PMCID
- PMC3367895
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Grant note
- National Institutes of Health
- Language
- English
- Date published
- 05/24/2012
- Academic Unit
- Pathology
- Record Identifier
- 9984047863902771
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