Journal article
Genome‐wide DNA methylation investigation of glucocorticoid exposure within buccal samples
Psychiatry and clinical neurosciences, Vol.73(6), pp.323-330
06/2019
DOI: 10.1111/pcn.12835
PMID: 30821055
Abstract
Aim: Glucocorticoids play a major role in regulating the stress response, and an imbalance of glucocorticoids has been implicated in stress-related disorders. Within mouse models, CpGs across the genome have been shown to be differentially methylated in response to glucocorticoid treatment, and using the Infinium 27K array, it was shown that humans given synthetic glucocorticoids had DNA methylation (DNAm) changes in blood. However, further investigation of the extent to which glucocorticoids affect DNAm across a larger proportion of the genome is needed.
Methods: Buccal samples were collected before and after synthetic glucocorticoid treatment in the context of a dental procedure. This included 30 tooth extraction surgery patients who received 10 mg of dexamethasone. Genome-wide DNAm was assessed with the Infinium HumanMethylationEPIC array.
Results: Five CpGs showed genome-wide significant DNAm changes that were >10%. These differentially methylated CpGs were in or nearest the following genes: ZNF438, KLHDC10, miR-544 or CRABP1, DPH5, and WDFY2. Using previously published datasets of human blood gene expression changes following dexamethasone exposure, a significant proportion of genes with false-discovery-rate-adjusted significant CpGs were also differentially expressed. A pathway analysis of the genes with false-discovery-rate-adjusted significant CpGs revealed significant enrichment of olfactory transduction, pentose and glucuronate interconversions, ascorbate and aldarate metabolism, and steroid hormone biosynthesis pathways.
Conclusion: High-dose synthetic glucocorticoid administration in the setting of a dental procedure was significantly associated with DNAm changes within buccal samples. These findings are consistent with prior findings of an influence of glucocorticoids on DNAm in humans.
Details
- Title: Subtitle
- Genome‐wide DNA methylation investigation of glucocorticoid exposure within buccal samples
- Creators
- Patricia R Braun - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USA, Department of Psychiatry and Behavioral ScienceSchool of Medicine, Johns Hopkins University Baltimore USAMai Tanaka‐Sahker - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USAAubrey C Chan - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USASydney S Jellison - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USAMason J Klisares - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USABenjamin W Hing - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USAYaseen Shabbir - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USALindsey N Gaul - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USAYasunori Nagahama - Department of NeurosurgeryCarver College of Medicine, University of Iowa Iowa City USAJulian Robles - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USAJonathan T Heinzman - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USASayeh Sabbagh - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USAEllyn M Cramer - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USAGabrielle N Duncan - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USAKumi Yuki - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USALiesl N Close - Department of NeurosurgeryCarver College of Medicine, University of Iowa Iowa City USABrian J Dlouhy - Department of NeurosurgeryCarver College of Medicine, University of Iowa Iowa City USAMatthew A Howard - Department of NeurosurgeryCarver College of Medicine, University of Iowa Iowa City USAHiroto Kawasaki - Department of NeurosurgeryCarver College of Medicine, University of Iowa Iowa City USAKyle M Stein - Department of Oral and Maxillofacial SurgeryCarver College of Medicine, University of Iowa Iowa City USAJames B Potash - Department of Psychiatry and Behavioral ScienceSchool of Medicine, Johns Hopkins University Baltimore USAGen Shinozaki - Department of PsychiatryCarver College of Medicine, University of Iowa Iowa City USA, Department of NeurosurgeryCarver College of Medicine, University of Iowa Iowa City USA, Iowa Neuroscience InstituteCarver College of Medicine, University of Iowa Iowa City USA, Interdisciplinary Graduate Program for NeuroscienceCarver College of Medicine, University of Iowa Iowa City USA
- Resource Type
- Journal article
- Publication Details
- Psychiatry and clinical neurosciences, Vol.73(6), pp.323-330
- DOI
- 10.1111/pcn.12835
- PMID
- 30821055
- ISSN
- 1323-1316
- eISSN
- 1440-1819
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: K23MH107654, T32GM008629
- Language
- English
- Date published
- 06/2019
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Psychiatry; Iowa Neuroscience Institute; Family and Community Medicine; Anesthesia; Oral and Maxillofacial Surgery; Neurosurgery; Otolaryngology; Internal Medicine
- Record Identifier
- 9984003455002771
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