Journal article
Genomewide Gene Expression Profiles of HPV-Positive and HPV-Negative Oropharyngeal Cancer Potential Implications for Treatment Choices
Archives of otolaryngology--head & neck surgery, Vol.135(2), pp.180-188
02/01/2009
DOI: 10.1001/archoto.2008.540
PMCID: PMC2761829
PMID: 19221247
Abstract
Objective: To study the difference in gene expression between human papillomavirus (HPV)-positive and HPV-negative oral cavity and oropharyngeal squamous cell carcinoma (OSCC).
Design: We used Affymetrix U133 plus 2.0 arrays to examine gene expression profiles of OSCC and normal oral tissue. The HPV DNA was detected using polymerase chain reaction followed by the Roche LINEAR ARRAY HPV Genotyping Test, and the differentially expressed genes were analyzed to examine their potential biological roles using the Ingenuity Pathway Analysis Software, version 5.0.
Setting: Three medical centers affiliated with the University of Washington.
Patients: A total of 119 patients with primary OSCC and 35 patients without cancer, all of whom were treated at the setting institutions, provided tissues samples for the study.
Results: Human papillomavirus DNA was found in 41 of 119 tumors (34.5%) and 2 of 35 normal tissue samples (5.7%); 39 of the 43 HPV specimens were HPV-16. A higher prevalence of HPV DNA was found in oropharyngeal cancer (23 of 31) than in oral cavity cancer (18 of 88). We found no significant difference in gene expression between HPV-positive and HPV-negative oral cavity cancer but found 446 probe sets (347 known genes) differentially expressed in HPV-positive oropharyngeal cancer than in HPV-negative oropharyngeal cancer. The most prominent functions of these genes are DNA replication, DNA repair, and cell cycling. Some genes differentially expressed between HPV-positive and HPV-negative oropharyngeal cancer (eg, TYMS, STMN1, CCND1, and RBBP4) are involved in chemotherapy or radiation sensitivity.
Conclusion: These results suggest that differences in the biology of HPV-positive and HPV-negative oropharyngeal cancer may have implications for the management of patients with these different tumors.
Details
- Title: Subtitle
- Genomewide Gene Expression Profiles of HPV-Positive and HPV-Negative Oropharyngeal Cancer Potential Implications for Treatment Choices
- Creators
- Pawadee Lohavanichbutr - Fred Hutch Cancer CenterJohn Houck - Fred Hutch Cancer CenterWenhong Fan - Fred Hutch Cancer CenterBevan Yueh - University of MinnesotaEduardo Mendez - University of WashingtonNeal Futran - University of WashingtonDavid R. Doody - Fred Hutch Cancer CenterMelissa P. Upton - University of WashingtonD. Gregory Farwell - University of California, DavisStephen M. Schwartz - University of WashingtonLue Ping Zhao - Fred Hutch Cancer CenterChu Chen - Fred Hutch Cancer Center
- Resource Type
- Journal article
- Publication Details
- Archives of otolaryngology--head & neck surgery, Vol.135(2), pp.180-188
- DOI
- 10.1001/archoto.2008.540
- PMID
- 19221247
- PMCID
- PMC2761829
- NLM abbreviation
- Arch Otolaryngol Head Neck Surg
- ISSN
- 0886-4470
- eISSN
- 1538-361X
- Publisher
- American Medical Association
- Number of pages
- 9
- Grant note
- R01CA095419 / National Cancer Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Language
- English
- Date published
- 02/01/2009
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984966854102771
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