Journal article
Genomic analysis of varicella-zoster virus: primers for individual open reading frames
Journal of clinical virology, Vol.28(1), pp.104-110
2003
DOI: 10.1016/S1386-6532(03)00073-8
PMID: 12927757
Abstract
The genome of varicella-zoster virus (VZV) contains nearly 125 000 bp. Preliminary genomic analysis has revealed that VZV may be less immutable than once thought. Through the investigation of the VZV genome using specifically designed oligonucleotides, it has been learned that sequence variation within VZV open reading frame 62 can distinguish between vaccine and wild-type virus. Additionally, the presence of single nucleotide polymorphisms within the VZV genome has identified distinct VZV populations originating from circumscribed geographic locations. In order for future studies of VZV genetic diversity to be carried out, amplifying and sequencing primers for individual VZV genes have been catalogued. Additionally, this report will facilitate the selection of VZV primers by which to distinguish clinical VZV isolates from vaccinia virus isolates.
Details
- Title: Subtitle
- Genomic analysis of varicella-zoster virus: primers for individual open reading frames
- Creators
- T.R Wagenaar - Departments of Microbiology and Pediatrics, University of Iowa Hospital/2501 JCP, 200 Hawkins Drive, Iowa City, IA 52242, USAC Grose - Departments of Microbiology and Pediatrics, University of Iowa Hospital/2501 JCP, 200 Hawkins Drive, Iowa City, IA 52242, USAV.N Loparev - U.S. Department of Health and Human Services, Division of Viral and Rickettsial Disease, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, GA, USAD.S Schmid - U.S. Department of Health and Human Services, Division of Viral and Rickettsial Disease, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, GA, USAJ Breuer - Department of Medical Microbiology, St. Bartholomew and the Royal London Hospital Medical Schools, Queen Mary and Westfield College, London, UK
- Resource Type
- Journal article
- Publication Details
- Journal of clinical virology, Vol.28(1), pp.104-110
- DOI
- 10.1016/S1386-6532(03)00073-8
- PMID
- 12927757
- NLM abbreviation
- J Clin Virol
- ISSN
- 1386-6532
- eISSN
- 1873-5967
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 2003
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093309602771
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