Journal article
Genomic and Epigenomic Evaluation of Electrically Induced Exercise in People With Spinal Cord Injury: Application to Precision Rehabilitation
Physical therapy, Vol.102(1), pzab243
01/01/2022
DOI: 10.1093/ptj/pzab243
PMCID: PMC8754383
PMID: 34718779
Abstract
Objective. Physical therapists develop patient-centered exercise prescriptions to help overcome the physical, emotional, psychosocial, and environmental stressors that undermine a person's health. Optimally prescribing muscle activity for people with disability, such as a spinal cord injury, is challenging because of their loss of volitional movement control and the deterioration of their underlying skeletal systems. This report summarizes spinal cord injury-specific factors that should be considered in patient-centered, precision prescription of muscle activity for people with spinal cord injury. This report also presents a muscle genomic and epigenomic analysis to examine the regulation of the proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 alpha) (oxidative) and myostatin (hypertrophy) signaling pathways in skeletal muscle during low-frequency (lower-force) electrically induced exercise versus higher-frequency (higher-force) electrically induced exercise under constant muscle recruitment (intensity).
Methods. Seventeen people with spinal cord injury participated in 1 or more unilateral electrically induced exercise sessions using a lower-force (1-, 3-, or 5-Hz) or higher-force (20-Hz) protocol. Three hours after the exercise session, percutaneous muscle biopsies were performed on exercised and nonexercised muscles for genomic and epigenomic analysis.
Results. We found that low-frequency (low-force) electrically induced exercise significantly increased the expression of PGC-1 alpha and decreased the expression of myostatin, consistent with the expression changes observed with high-frequency (higher-force) electrically induced exercise. Further, we found that low-frequency (lower-force) electrically induced exercise significantly demethylated, or epigenetically promoted, the PGC-1 alpha signaling pathway. A global epigenetic analysis showed that >70 pathways were regulated with low-frequency (lower-force) electrically induced exercise.
Conclusion. These novel results support the notion that low-frequency (low-force) electrically induced exercise may offer a more precise rehabilitation strategy for people with chronic paralysis and severe osteoporosis. Future clinical trials are warranted to explore whether low-frequency (lower-force) electrically induced exercise training affects the overall health of people with chronic spinal cord injury.
Details
- Title: Subtitle
- Genomic and Epigenomic Evaluation of Electrically Induced Exercise in People With Spinal Cord Injury: Application to Precision Rehabilitation
- Creators
- Michael A. Petrie - Roy J. and Lucille A. Carver College of MedicineEric B. Taylor - Roy J. and Lucille A. Carver College of MedicineManish Suneja - Roy J. and Lucille A. Carver College of MedicineRichard K. Shields - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Physical therapy, Vol.102(1), pzab243
- DOI
- 10.1093/ptj/pzab243
- PMID
- 34718779
- PMCID
- PMC8754383
- NLM abbreviation
- Phys Ther
- ISSN
- 0031-9023
- eISSN
- 1538-6724
- Publisher
- Oxford Univ Press
- Number of pages
- 11
- Grant note
- R01HD084645; R01HD082109 / National Institute of Child Health and Human Development; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) R00 AR059190 / National Institute of Arthritis and Musculoskeletal and Skin Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) University of Iowa R01 DK104998 / National Institute of Diabetes and Digestive and Kidney Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) UL1TR002537 / National Center for Advancing Translational Sciences Research
- Language
- English
- Date published
- 01/01/2022
- Academic Unit
- Molecular Physiology and Biophysics; Orthopedics and Rehabilitation; Physical Therapy and Rehabilitation Science; Fraternal Order of Eagles Diabetes Research Center; Nephrology; Internal Medicine
- Record Identifier
- 9984294958202771
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