Journal article
Genomic biomarkers in chronic beryllium disease and sarcoidosis
Respiratory medicine, Vol.187, pp.106390-106390
10/2021
DOI: 10.1016/j.rmed.2021.106390
PMCID: PMC8490480
PMID: 34399367
Abstract
Background Previous gene expression studies have identified genes IFNγ, TNFα, RNase 3, CXCL9, and CD55 as potential biomarkers for sarcoidosis and/or chronic beryllium disease (CBD). We hypothesized that differential expression of these genes could function as diagnostic biomarkers for sarcoidosis and CBD, and prognostic biomarkers for sarcoidosis. Study Design/Methods We performed RT-qPCR on whole blood samples from CBD (n = 132), beryllium sensitized (BeS) (n = 109), and sarcoidosis (n = 99) cases and non-diseased controls (n = 97) to determine differential expression of target genes. We then performed logistic regression modeling and generated ROC curves to determine which genes could most accurately differentiate: 1) CBD versus sarcoidosis 2) CBD versus BeS 3) sarcoidosis versus controls 4) non-progressive versus progressive sarcoidosis. Results CD55 and TNFα were significantly upregulated, while CXCL9 was significantly downregulated in CBD compared to sarcoidosis (p < 0.05). The ROC curve from the logistic regression model demonstrated high discriminatory ability of the combination of CD55, TNFα, and CXCL9 to distinguish between CBD and sarcoidosis with an AUC of 0.98. CD55 and TNFα were significantly downregulated in sarcoidosis compared to controls (p < 0.05). The ROC curve from the model showed a reasonable discriminatory ability of CD55 and TNFα to distinguish between sarcoidosis and controls with an AUC of 0.86. There was no combination of genes that could accurately differentiate between CBD and BeS or sarcoidosis phenotypes. Interpretation CD55, TNFα and CXCL9 expression levels can accurately differentiate between CBD and sarcoidosis, while CD55 and TNFα expression levels can accurately differentiate sarcoidosis and controls.
•Genomic biomarkers used as a diagnostic tool in sarcoidosis and CBD.•CD55, TNFα, and CXCL9 gene levels distinguish between sarcoidosis and CBD.•CD55 and TNFα gene levels distinguish between sarcoidosis and controls.
Details
- Title: Subtitle
- Genomic biomarkers in chronic beryllium disease and sarcoidosis
- Creators
- Nancy W. Lin - University of Colorado DenverLisa A. Maier - Colorado School of Public HealthMargaret M. Mroz - National Jewish HealthSean Jacobson - National Jewish HealthKristyn MacPhail - National Jewish HealthSucai Liu - National Jewish HealthZhe Lei - Soochow UniversityBriana Q. Barkes - National Jewish HealthTasha E. Fingerlin - Colorado School of Public HealthNabeel Hamzeh - University of IowaAnnyce S. Mayer - Colorado School of Public HealthClara I. Restrepo - National Jewish HealthDivya Chhabra - National Jewish HealthIvana V. Yang - University of Colorado DenverLi Li - University of Colorado Denver
- Resource Type
- Journal article
- Publication Details
- Respiratory medicine, Vol.187, pp.106390-106390
- DOI
- 10.1016/j.rmed.2021.106390
- PMID
- 34399367
- PMCID
- PMC8490480
- NLM abbreviation
- Respir Med
- ISSN
- 0954-6111
- eISSN
- 1532-3064
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health; DOI: 10.13039/100000050, name: National Heart Lung and Blood Institute; DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences
- Language
- English
- Date published
- 10/2021
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984359575002771
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