Genomic organization of the 5' end of human beta-ENaC and preliminary characterization of its promoter

Christie P Thomas; Randy W Loftus; Kang Z Liu; Omar A Itani

doi:10.1152/ajprenal.00268.2001

Back

Genomic organization of the 5' end of human beta-ENaC and preliminary characterization of its promoter

Journal article

Open access

Peer reviewed

Genomic organization of the 5' end of human beta-ENaC and preliminary characterization of its promoter

Christie P Thomas, Randy W Loftus, Kang Z Liu and Omar A Itani

American journal of physiology. Renal physiology, Vol.282(5), pp.F898-909

05/2002

DOI: 10.1152/ajprenal.00268.2001

PMID: 11934701

Files and links (1)

url

https://doi.org/10.1152/ajprenal.00268.2001View

Published (Version of record) Open Access

Abstract

The mRNA for the beta-subunit of the epithelial Na(+) channel (beta-ENaC) is regulated developmentally and, in some tissues, in response to corticosteroids. To understand the mechanisms of transcriptional regulation of the human beta-ENaC gene, we characterized the 5' end of the gene and its 5'-flanking regions. Adaptor-ligated human kidney and lung cDNA were amplified by 5' rapid amplification of cDNA ends, and transcription start sites of two 5' variant transcripts were determined by nuclease protection or primer extension assays. Cosmid clones that contain the 5' end of the gene were isolated, and analysis of these clones indicated that alternate first exons approximately 1.5 kb apart and approximately 45 kb upstream of a common second exon formed the basis of these transcripts. Genomic fragments that included the proximal 5'-flanking region of either transcript were able to direct expression of a reporter gene in lung epithelia and to bind Sp1 in nuclear extracts, confirming the presence of separate promoters that regulate beta-ENaC expression.

DNA, Complementary - analysis

Amino Acid Sequence

Cosmids

Promoter Regions, Genetic

Protein Biosynthesis

Gene Library

Exons

Humans

Gene Expression Regulation

Lung - chemistry

Molecular Sequence Data

RNA, Messenger - analysis

Immunoblotting

Sp1 Transcription Factor - metabolism

Epithelial Sodium Channels

Gene Amplification

Transfection

Sodium Channels - chemistry

Base Sequence

Cloning, Molecular

Kidney - chemistry

Transcription, Genetic

Sodium Channels - genetics

Details

Title: Subtitle: Genomic organization of the 5' end of human beta-ENaC and preliminary characterization of its promoter
Creators: Christie P Thomas - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242-1081, USA. christie-thomas@uiowa.edu
Randy W Loftus
Kang Z Liu
Omar A Itani
Resource Type: Journal article
Publication Details: American journal of physiology. Renal physiology, Vol.282(5), pp.F898-909
DOI: 10.1152/ajprenal.00268.2001
PMID: 11934701
NLM abbreviation: Am J Physiol Renal Physiol
ISSN: 1931-857X
eISSN: 1522-1466
Publisher: United States
Grant note: R01 DK054348 / NIDDK NIH HHS DK-54348 / NIDDK NIH HHS
Language: English
Date published: 05/2002
Academic Unit: Stead Family Department of Pediatrics; Anesthesia; Obstetrics and Gynecology; Nephrology; Internal Medicine
Record Identifier: 9983986375402771

Metrics

17 Record Views

17 Times Cited - Web of Science