Journal article
Genotypic characterization and comparison of Streptococcus mutans in American Indian and Southeast Iowa children
Clinical and Experimental Dental Research, Vol.3(6), pp.235-243
12/01/2017
DOI: 10.1002/cre2.94
PMCID: PMC5788224
PMID: 29416899
Abstract
Early childhood caries is a complex health care issue that has a multifactorial etiology. One aspect of this etiology is the colonization and propagation of acidogenic bacteria at an early age. There have been several bacterial species associated with caries but 1 common species is Streptococcus mutans. Here, we describe genotypic diversity and commonality of Streptococcus mutans recovered from children representing 2 groups with similar socioeconomic demographics: a Northern Plains American Indian Tribe and a Southeast Iowa population. Forty 36‐month‐old American Indian children were selected from a cohort of 239 mothers and children, and forty 2‐ to 5‐year‐old children from Southeast Iowa were selected to compare the genotypic profiles of Streptococcus mutans recovered from each child's plaque. S. mutans isolates were selected from whole mouth plaque samples; DNA was extracted and amplified via AP‐PCR to show specific genotype patterns. These patterns were compared with GelComparIIv6.5 gel analysis software. We found 18 distinct genotypes from 524 isolates; 13 of which were common between the 2 communities. Five genotypes were unique to only the American Indian children while the Southeast Iowa children harbored no unique genotypes. Although the American Indian children had some genotypes that were not present in the Southeast Iowa children, these were not widely distributed among the community. Furthermore, the levels of genotypic diversity and commonality were similar between the 2 populations. This study sets the groundwork for a comprehensive comparison of genotypes and caries among larger subsections of both populations.
Details
- Title: Subtitle
- Genotypic characterization and comparison of Streptococcus mutans in American Indian and Southeast Iowa children
- Creators
- Alissa L. Villhauer - University of Iowa, Dental ResearchDavid J. Lynch - University of IowaJohn J. Warren - University of Iowa, Preventive and Community DentistryDeborah V. Dawson - University of Iowa, Dental ResearchDerek R. Blanchette - University of IowaDavid R Drake - University of Iowa, Dental Research
- Resource Type
- Journal article
- Publication Details
- Clinical and Experimental Dental Research, Vol.3(6), pp.235-243
- Publisher
- John Wiley & Sons, Inc; Hoboken
- DOI
- 10.1002/cre2.94
- PMID
- 29416899
- PMCID
- PMC5788224
- ISSN
- 2057-4347
- eISSN
- 2057-4347
- Copyright
- ©2017 The Authors.
- Grant note
- Funding information: Roy J. Carver Charitable Trust, Grant/AwardNumber: 06‐2336; National Institutes ofHealth, Grant/Award Number: R01‐DE017736
- Language
- English
- Date published
- 12/01/2017
- Academic Unit
- Endodontics
- Record Identifier
- 9983763492102771
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