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Ghost vessels in the eye: Cell free choriocapillaris domains in atrophic age-related macular degeneration
Journal article   Peer reviewed

Ghost vessels in the eye: Cell free choriocapillaris domains in atrophic age-related macular degeneration

Robert F. Mullins, Miles J. Flamme-Wiese, Emma M. Navratil, Erin A. Boese, Katayoun Varzavand, Megan J. Riker, Kai Wang, Edwin M. Stone and Budd A. Tucker
Experimental eye research, Vol.248, 110128
11/2024
DOI: 10.1016/j.exer.2024.110128
PMCID: PMC11532014
PMID: 39419369

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Abstract

The choriocapillaris is a dense vascular bed in the inner choroid that supplies the photoreceptor cells and retinal pigment epithelium (RPE). While loss of choriocapillaris density has been described in association with age-related macular degeneration (AMD), whether these changes are primary or secondary to RPE degenerative changes in AMD has been debated. In this study we characterized choriocapillaris loss by quantifying “ghost” vessels in a series of 99 human donor maculae labeled with the UEA-I lectin, and found significant increases in early-intermediate AMD and a greater difference in geographic atrophy in areas with intact RPE. Eyes were genotyped at the CFH Tyr402His locus, and those homozygous for the His allele showed significantly more ghost vessels than those with other genotypes. When only non-AMD eyes were evaluated, His homozygotes had increased ghost vessel density but this trend did not reach statistical significance. These results support the notion that choriocapillaris death often precedes RPE degeneration in AMD and that this loss is an important therapeutic consideration for AMD. •Ghost vessels in the choriocapillaris are nonperfused lumens that persist after endothelial death.•Density of ghost vessels was measured in human donor maculae with early to advanced AMD and controls.•Genotyping was also performed at the CFH Y402H locus.•Advancing AMD and to a lesser extent genotype was associated with density of ghost vessels.•Relevance to therapy is discussed.
Aging Age-related macular degeneration Choriocapillaris

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