Journal article
Giant Drosophila neurons differentiated from cytokinesis-arrested embryonic neuroblasts
Journal of neurobiology, Vol.21(3), pp.499-507
04/1990
DOI: 10.1002/neu.480210310
PMID: 2112589
Abstract
The relative contributions of the intrinsic and extrinsic factors in determining neuronal differentiation are not fully understood yet. We found that isolated neuroblasts from Drosophila gastrulae were able to differentiate neuron-specific properties in culture even when cell divisions were inhibited. The resultant giant multinucleated neurons displayed thickened neurites with a variety of distinct branching patterns. Neuronal antigens were expressed as in normal cultured neurons, and action potentials could be evoked by current injection within two days after plating. These results indicate that the factors for initiating specific differentiation programs for basic neuronal form and function are present in a neuroblast already. The cells of increased sizes in this culture system are more accessible to physiological and cell biological analyses and could facilitate future studies of the Drosophila nervous system.
Details
- Title: Subtitle
- Giant Drosophila neurons differentiated from cytokinesis-arrested embryonic neuroblasts
- Creators
- Chun‐Fang WuKuniyoshi SakaiMitsuyoshi SaitoYoshiki Hotta
- Resource Type
- Journal article
- Publication Details
- Journal of neurobiology, Vol.21(3), pp.499-507
- DOI
- 10.1002/neu.480210310
- PMID
- 2112589
- NLM abbreviation
- J Neurobiol
- ISSN
- 0022-3034
- eISSN
- 1097-4695
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- Number of pages
- 9
- Language
- English
- Date published
- 04/1990
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070536302771
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