Journal article
GigC, a LysR Family Transcription Regulator, Is Required for Cysteine Metabolism and Virulence in Acinetobacter baumannii
Infection and immunity, Vol.89(1), e00180-20
01/01/2021
DOI: 10.1128/IAI.00180-20
PMCID: PMC7927930
PMID: 33077621
Abstract
A critical facet of mammalian innate immunity involves the hosts' attempts to sequester and/or limit the availability of key metabolic products from pathogens. For example, nutritional immunity encompasses host approaches to limit the availability of key heavy metal ions such as zinc and iron. Previously, we identified several hundred genes in a multidrug-resistant isolate of Acinetobacter baumannii that are required for growth and/or survival in the Galleria mellonella infection model. In the present study, we further characterize one of these genes, a LysR family transcription regulator that we previously named GigC. We show that mutant strains lacking gigC have impaired growth in the absence of the amino acid cysteine and that gigC regulates the expression of several genes involved in the sulfur assimilation and cysteine biosynthetic pathways. We further show that cells harboring a deletion of the gigC gene are attenuated in two murine infection models, suggesting that the GigC protein, likely through its regulation of the cysteine biosynthetic pathway, plays a key role in the virulence of A. baumannii.
Details
- Title: Subtitle
- GigC, a LysR Family Transcription Regulator, Is Required for Cysteine Metabolism and Virulence in Acinetobacter baumannii
- Creators
- Michael J. Gebhardt - Boston Children's HospitalDaniel M. Czyz - University of FloridaShweta Singh - Walter Reed Army Institute of ResearchDaniel Zurawski - Walter Reed Army Institute of ResearchLev Becker - University of ChicagoHoward A. Shuman - University of Chicago
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.89(1), e00180-20
- Publisher
- Amer Soc Microbiology
- DOI
- 10.1128/IAI.00180-20
- PMID
- 33077621
- PMCID
- PMC7927930
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Number of pages
- 13
- Grant note
- 1R21AI115203 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Cystic Fibrosis Foundation; Italian Cystic Fibrosis Research Foundation
- Language
- English
- Date published
- 01/01/2021
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984297333002771
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