Journal article
Ginsenoside Rg1 attenuates hypoxia and hypercapnia-induced vasoconstriction in isolated rat pulmonary arterial rings by reducing the expression of p38
Journal of thoracic disease, Vol.8(7), pp.1513-1523
07/01/2016
DOI: 10.21037/jtd.2016.05.71
PMCID: PMC4958841
PMID: 27499938
Abstract
Background: Pulmonary arterial hypertension (PAH) is a fatal disease characterized by increased pulmonary arteriolar resistance. Pulmonary vasoconstriction has been proved to play a significant role in PAH. We previously reported that Panax notoginseng saponins (PNS) might attenuate hypoxia and hypercapnia-induced pulmonary vasoconstriction (HHPV).
Methods: In the present study, our specific objective was to investigate the role of ginsenoside Rg1, a major component of PNS, in this process and the possible underlying mechanism. The second order pulmonary rings isolated from the Sprague-Dawley rats were treated with different dosage of ginsenoside Rg1 at 8, 40, or 100 mg/L respectively, both before and during the conditions of hypoxia and hypercapnia. Contractile force changes of the rings were detected. Furthermore, SB203580, the selective inhibitor for p38 activation was applied to the rings. Pulmonary arterial smooth muscle cells (PASMCs) were cultured under hypoxic and hypercapnic conditions, and ginsenoside Rg1 was administered to detect the changes induced by p38.
Results: Under the hypoxic and hypercapnic conditions, we observed a biphasic pulmonary artery contractile response to the second pulmonary artery rings. It is hypothesized that the observed attenuation of vasoconstriction and the production of vasodilation could have been induced by ginsenoside Rg1. This effect was significantly reinforced by SB203580 (P<0.05 or P<0.01). The expression of p38 in the PASMCs under hypoxic and hypercapnic conditions was significantly activated (P<0.05 or P<0.01) and the observed activation was attenuated by ginsenoside Rg1 (P<0.05 or P<0.01).
Conclusions: Our findings strongly support the significant role of ginsenoside Rg1 in the inhibition of hypoxia and hypercapnia-induced vasoconstriction by the p38 pathway.
Details
- Title: Subtitle
- Ginsenoside Rg1 attenuates hypoxia and hypercapnia-induced vasoconstriction in isolated rat pulmonary arterial rings by reducing the expression of p38
- Creators
- Mengxiao Zheng - Wenzhou Medical UniversityMeiping Zhao - Wenzhou Med Univ, Dept Pathophysiol, Wenzhou 325035, Peoples R ChinaLanlan Tang - Wenzhou Medical UniversityCongcong Zhang - Wenzhou Med Univ, Dept Pathophysiol, Wenzhou 325035, Peoples R ChinaLongsheng Song - Univ Iowa, Carver Coll Med, Div Cardiovasc Med, Iowa City, IA 52242 USAWantie Wang - Wenzhou Medical University
- Resource Type
- Journal article
- Publication Details
- Journal of thoracic disease, Vol.8(7), pp.1513-1523
- DOI
- 10.21037/jtd.2016.05.71
- PMID
- 27499938
- PMCID
- PMC4958841
- NLM abbreviation
- J Thorac Dis
- ISSN
- 2072-1439
- eISSN
- 2077-6624
- Publisher
- Ame Publ Co
- Number of pages
- 11
- Grant note
- 2012-XK-A28 / Key Construction Academic Subject (Traditional Chinese Medicine) of Zhejiang Province 2008ZA017; 2013ZZ011 / key project of Traditional Chinese Medicine Development Plan of Zhejiang province
- Language
- English
- Date published
- 07/01/2016
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984288732002771
Metrics
18 Record Views