Journal article
Gliclazide Attenuates the Intracellular Ca2+ Changes Induced In Vitro by Ischemia in the Retinal Slices of Rats with Streptozotocin-Induced Diabetes
Current eye research, Vol.30(9), pp.789-798
2005
DOI: 10.1080/02713680591002808
PMID: 16146925
Abstract
Purpose: To investigate the dynamics of the intracellular Ca2+ concentration ([Ca2+]i) during retinal ischemia in rats with streptozotocin (STZ)-induced diabetes and the effect of gliclazide, a sulfonylurea with a potent free-radical scavenging activity on ischemia-induced [Ca2+]i dynamics. Methods: Rats with STZ (65 mg/kg) induced diabetes were divided into three groups: the untreated diabetic group, the gliclazide-treated group, and the glibenclamide-treated group. An ischemic condition was imposed in vitro on the retinal slices by perfusion with an oxygen/glucose deprived solution. The [Ca2+]i was measured in individual layers of the rat retinal slices loaded with the Ca2+ indicator fluo-3. Results: As compared to that in the normal rat retina, both the amplitude and the kinetics of the [Ca2+]i increase were suppressed in the intermediate layers of the retinal slices from the diabetic rats under the ischemic condition. These changes were attenuated by the administration of gliclazide but not by that of glibenclamide. Conclusions: Hyperglycemia influences ischemia-induced [Ca2+]i dynamics predominantly in the intermediate layers of the retina, and gliclazide, as compared to glibenclamide without a free radical scavenging activity, potently attenuates the ischemia-induced changes in the calcium dynamics.
Details
- Title: Subtitle
- Gliclazide Attenuates the Intracellular Ca2+ Changes Induced In Vitro by Ischemia in the Retinal Slices of Rats with Streptozotocin-Induced Diabetes
- Creators
- Jiro Kinukawa - 1Department of Ophthalmology and Medical Science, Tohoku University Graduate School of Medicine, Sendai MiyagiJapanMasahiko Shimura - 1Department of Ophthalmology and Medical Science, Tohoku University Graduate School of Medicine, Sendai MiyagiJapanNobutoshi Harata - 3Department of Molecular and Cellular Physiology, Beckman Center, Stanford University School of Medicine, Stanford, California, USAMakoto Tamai - 1Department of Ophthalmology and Medical Science, Tohoku University Graduate School of Medicine, Sendai MiyagiJapan
- Resource Type
- Journal article
- Publication Details
- Current eye research, Vol.30(9), pp.789-798
- Publisher
- Informa UK Ltd
- DOI
- 10.1080/02713680591002808
- PMID
- 16146925
- ISSN
- 0271-3683
- eISSN
- 1460-2202
- Language
- English
- Date published
- 2005
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984025677102771
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