Journal article
Global Igfbp1 deletion does not affect prostate cancer development in a c-Myc transgenic mouse model
Journal of endocrinology, Vol.211(3), pp.297-304
12/01/2011
DOI: 10.1530/JOE-11-0240
PMID: 21903863
Abstract
Circulating insulin-like growth factor binding protein 1 (IGFBP1) levels vary in response to nutritional status, and pre-clinical studies suggest that elevated IGFBP1 may be protective against the development and progression of prostate cancer. We hypothesized that global deletion of Igfbp1 would accelerate the development of prostate cancer in a c-Myc transgenic mouse model. To test our hypothesis, c-Myc transgenic mice (Myc/BP-1 wild-type (WT)) were crossed and interbred with the Igfbp1 knockout mice (Myc/BP-1 KO). The animals were placed on a high-protein diet at weaning, weighed every 2 weeks, and euthanized at 16 weeks of age. Prostate histopathology was assessed and proliferation status was determined by Ki-67 and proliferating cell nuclear antigen analyses. IGF-related serum biomarkers and body composition were measured. No significant difference in the incidence of prostate cancer was observed between the Myc/BP-1 KO and the Myc/BP-1 WT mice (65 and 80% respectively, P=0.48). Proliferation was significantly decreased by 71% in prostate tissue of Myc/BP-1 KO mice compared with Myc/BP-1 WT mice. Myc/BP-1 KO mice exhibited a significant 6.7% increase in body weight relative to the Myc/BP-1 WT mice that was attributed to an increase in fat mass. Fasting insulin levels were higher in the Myc/BP-1 KO mice without any difference between the groups in fasting glucose concentrations. Thus, contrary to our hypothesis, global deletion of Igfbp1 in a c-Myc transgenic mouse model did not accelerate the development of prostate cancer. Global Igfbp1 deletion did result in a significant increase in body weight and body fat mass. Further studies are required to understand the underlying mechanisms for these metabolic effects. Journal of Endocrinology (2011) 211, 297-304
Details
- Title: Subtitle
- Global Igfbp1 deletion does not affect prostate cancer development in a c-Myc transgenic mouse model
- Creators
- Ashley Gray - University of California, Los AngelesWilliam J. Aronson - Univ Calif Los Angeles, David Geffen Sch Med, Dept Urol, Los Angeles, CA 90095 USAR. James Barnard - University of California, Los AngelesHemal Mehta - Univ Calif Los Angeles, David Geffen Sch Med, Div Pediat Endocrinol, Los Angeles, CA 90095 USAJunxiang Wan - Univ Calif Los Angeles, David Geffen Sch Med, Div Pediat Endocrinol, Los Angeles, CA 90095 USAJonathan Said - University of California, Los AngelesPinchas Cohen - Univ Calif Los Angeles, David Geffen Sch Med, Div Pediat Endocrinol, Los Angeles, CA 90095 USAColette Galet - Univ Calif Los Angeles, David Geffen Sch Med, Dept Urol, Los Angeles, CA 90095 USA
- Resource Type
- Journal article
- Publication Details
- Journal of endocrinology, Vol.211(3), pp.297-304
- DOI
- 10.1530/JOE-11-0240
- PMID
- 21903863
- NLM abbreviation
- J Endocrinol
- ISSN
- 0022-0795
- eISSN
- 1479-6805
- Publisher
- Bioscientifica Ltd
- Number of pages
- 8
- Grant note
- P50CA92131 / National Cancer Institute (NCI); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) P50CA092131 / National Cancer Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 2P30DK063491 / National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) 1P01AG034906 / National Institute of Aging (NIA); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA) Department of Veterans Affairs; US Department of Veterans Affairs
- Language
- English
- Date published
- 12/01/2011
- Academic Unit
- Injury Prevention Research Center; University of Iowa Health Care
- Record Identifier
- 9985138031502771
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