Gluconeogenesis in rabbit liver. III. The influences of glucagon, epinephrine, alpha- and beta-adrenergic agents on gluconeogenesis in isolated hepatocytes

Mark A Yorek; Gerald A Rufo Jr; Paul D Ray

doi:10.1016/0304-4165(80)90328-1

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Gluconeogenesis in rabbit liver. III. The influences of glucagon, epinephrine, alpha- and beta-adrenergic agents on gluconeogenesis in isolated hepatocytes

Journal article

Peer reviewed

Gluconeogenesis in rabbit liver. III. The influences of glucagon, epinephrine, alpha- and beta-adrenergic agents on gluconeogenesis in isolated hepatocytes

Mark A Yorek, Gerald A Rufo Jr and Paul D Ray

Biochimica et biophysica acta, Vol.632(4), pp.517-526

11/03/1980

DOI: 10.1016/0304-4165(80)90328-1

PMID: 6254576

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Abstract

1. Gluconeogenesis from various substrates has been demonstrated in hepatocytes from 48 h fasted rabbits. Maximum rates of gluconeogenesis (expressed as mumol glucose formed/30 min per 10(8) cells) are: D-fructose, 9.86; dihydroxyacetone, 5.28; L-lactate, 5.26; L-lactate/pyruvate, 3.83; pyruvate, 3.32; glycerol, 2.92; L-alanine, 2.24. 2. Gluconeogenesis from L-lactate is enhanced 1.3--1.5-fold over control values by glucagon, L-epinephrine, L-norepinephrine, dibutyryl cyclic AMP, L-phenylephrine and L-isoproterenol. Glucogenesis from both dihydroxyacetone and D-fructose is stimulated 1.7--2.0-fold of control values by glucagon, epinephrine and dibutyryl cyclic AMP. 3. Gluconeogenesis from lactate is enhanced by both alpha- and beta-adrenergic stimulations based on findings with alpha- and beta-agonists and antagonists. 4. Enhancement of gluconeogenesis by epinephrine and norepinephrine is apparently due to both alpha- and beta-adrenergic effects, as either propranolol or phentolamine partially inhibits such enhancement. The consistently more pronounced inhibition produced by propranolol implies that stimulation of glucose formation by catecholamines is more strongly beta-adrenergic related. Epinephrine-induced glycogenolysis in rabbit hepatocytes is severely inhibited by propranolol but insensitive to phentolamine, suggesting that glycogen breakdown is solely beta-adrenergic related. These observations contrast with those of others that stimulation of both gluconeogenesis and glycogenolysis by catecholamines while sensitive to both alpha- and beta-adrenergic stimulation in rats, at least young rats, is primarily alpha-adrenergic mediated, especially in adult rats.

Rabbits

Gluconeogenesis - drug effects

Liver - metabolism

Male

Phentolamine - pharmacology

Glucagon - pharmacology

Propranolol - pharmacology

Animals

Phenylephrine - pharmacology

Liver - drug effects

Isoproterenol - pharmacology

Bucladesine - pharmacology

Kinetics

In Vitro Techniques

Epinephrine - pharmacology

Details

Title: Subtitle: Gluconeogenesis in rabbit liver. III. The influences of glucagon, epinephrine, alpha- and beta-adrenergic agents on gluconeogenesis in isolated hepatocytes
Creators: Mark A Yorek
Gerald A Rufo Jr
Paul D Ray
Resource Type: Journal article
Publication Details: Biochimica et biophysica acta, Vol.632(4), pp.517-526
DOI: 10.1016/0304-4165(80)90328-1
PMID: 6254576
ISSN: 0006-3002
eISSN: 1878-2434
Grant note: AM 12705 / NIADDK NIH HHS
Language: English
Date published: 11/03/1980
Academic Unit: Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984094583802771

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