Journal article
Gluconeogenesis in rabbit liver: IV. The effects of glucagon, epinephrine, α-and β-adrenergic agents on gluconeogenesis and pyruvate kinase in hepatocytes given dihydroxyacetone or fructose
Biochimica et biophysica acta. General subjects, Vol.675(3), pp.309-315
1981
DOI: 10.1016/0304-4165(81)90019-2
PMID: 6268188
Abstract
1. Epinephrine, isoproterenol and phenylephrine each increases significantly gluconeogenesis (from dihydroxyacetone or
D-fructose) and glycogenolysis when added to hepatocytes from 48-h fasted rabbits. Such stimulation of both processes by epinephrine, isoproterenol or phenylephrine is negated by the β-adrenergic antagonist propranolol but remains significant in the presence of the α-adrenergic antagonist phentolamine. Conversely, previous data suggest that catecholamine-induced stimulation of glucose formation from
L-lactate in both α- and β-adrenergic-sensitive. 2. Glucagon, epinephrine, isoproterenol, phenylephrine and dibutyryl cyclic AMP each inhibits significantly pyruvate kinase activity in rabbit hepatocytes. Inhibition of pyruvate kinase activity by epinephrine, isoproterenol or phenylephrine is negated by propranolol but insensitive to phentolamine. 3. These observations suggest that enhancement by epinephrine of glucose formation from either dihydroxyacetone or
D-fructose is solely β-adrenergic-regulated, just as is its inhibition of pyruvate kinase activity. Stimulation of gluconeogenesis by glucagon, epinephrine, isoproterenol, phenylephrine or dibutyryl cyclic AMP may be at least in part directly related to their ability to inhibit pyruvate kinase.
Details
- Title: Subtitle
- Gluconeogenesis in rabbit liver: IV. The effects of glucagon, epinephrine, α-and β-adrenergic agents on gluconeogenesis and pyruvate kinase in hepatocytes given dihydroxyacetone or fructose
- Creators
- Mark A Yorek - Guy and Bertha Ireland Research Laboratory, Department of Biochemistry, University of North Dakota School of Medicine, Grand Forks, ND 58202, USAGerald A Rufo - Guy and Bertha Ireland Research Laboratory, Department of Biochemistry, University of North Dakota School of Medicine, Grand Forks, ND 58202, USAJames B Blair - Department of Biochemistry, West Virginia University Medical Center, Morgantown, WV 26506 U.S.APaul D Ray - Guy and Bertha Ireland Research Laboratory, Department of Biochemistry, University of North Dakota School of Medicine, Grand Forks, ND 58202, USA
- Resource Type
- Journal article
- Publication Details
- Biochimica et biophysica acta. General subjects, Vol.675(3), pp.309-315
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0304-4165(81)90019-2
- PMID
- 6268188
- ISSN
- 0304-4165
- eISSN
- 1872-8006
- Language
- English
- Date published
- 1981
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094605402771
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