Journal article
Glucose metabolism heterogeneity in human and mouse malignant glioma cell lines
Journal of neuro-oncology, Vol.74(2), pp.123-133
2005
DOI: 10.1007/s11060-004-6404-6
PMID: 16193382
Abstract
The current study examined specific bioenergetic markers associated with the metabolic phenotype of several human and mouse glioma cell lines. Based on preliminary studies, we hypothesized that glioma cells would express one of at least two different metabolic phenotypes, possibly acquired through progression. The D-54MG and GL261 glioma cell lines displayed an oxidative phosphorylation (OXPHOS)-dependent phenotype, characterized by extremely long survival under glucose starvation, and low tolerance to poisoning of the electron transport chain (ETC). Alternatively, U-251MG and U-87MG glioma cells exhibited a glycolytic-dependent phenotype with functional OXPHOS. These cells displayed low tolerance to glucose starvation and were resistant to a ETC blocker. Moreover, these cells could be rescued in low glucose conditions by oxidative substrates (e.g., lactate, pyruvate). Finally, these two phenotypes could be distinguished by the differential expression of LDH isoforms. OXPHOS-dependent cells expressed both LDH-A and -B isoforms whereas glycolytic-dependent glioma cells expressed only LDH-B. In the latter case, LDH-B would be expected to be essential for the use of extracellular lactate to fuel cell activities. These observations raise the possibility that the heterogeneity in glucose metabolism and, in particular, the sole expression of LDH-B, might identify an important biological marker of glioma cells that is critical for their progression and that might afford a new target for anticancer drugs.
Details
- Title: Subtitle
- Glucose metabolism heterogeneity in human and mouse malignant glioma cell lines
- Creators
- Corinne E GRIGUER - Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, United StatesClaudia R OLIVA - Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, United StatesG. Yancey GILLESPIE - Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, United States
- Resource Type
- Journal article
- Publication Details
- Journal of neuro-oncology, Vol.74(2), pp.123-133
- Publisher
- Springer; Dordrecht
- DOI
- 10.1007/s11060-004-6404-6
- PMID
- 16193382
- ISSN
- 0167-594X
- eISSN
- 1573-7373
- Language
- English
- Date published
- 2005
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047677302771
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