Glutathione Plays a Positive Role in the Proliferation of Pinus koraiensis Embryogenic Cells

Fang Gao; Chunxue Peng; Yue Zhang; Hao Wang; Hailong Shen; Ling Yang

doi:10.3390/ijms232314679

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Glutathione Plays a Positive Role in the Proliferation of Pinus koraiensis Embryogenic Cells

Journal article

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Glutathione Plays a Positive Role in the Proliferation of Pinus koraiensis Embryogenic Cells

Fang Gao, Chunxue Peng, Yue Zhang, Hao Wang, Hailong Shen and Ling Yang

International journal of molecular sciences, Vol.23(23), p.14679

12/01/2022

DOI: 10.3390/ijms232314679

PMCID: PMC9736457

PMID: 36499020

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Abstract

In the large-scale breeding of conifers, cultivating embryogenic cells with good proliferative capacity is crucial in the process of somatic embryogenesis. In the same cultural environment, the proliferative capacity of different cell lines is significantly different. To reveal the regulatory mechanism of proliferation in woody plant cell lines with different proliferative potential, we used Korean pine cell lines with high proliferative potential 001#–001 (Fast) and low proliferative potential 001#–010 (Slow) for analysis. A total of 17 glutathione-related differentially expressed genes was identified between F and S cell lines. A total of 893 metabolites was obtained from the two cell lines in the metabolomic studies. A total of nine metabolites related to glutathione was significantly upregulated in the F cell line compared with the S cell line. The combined analyses revealed that intracellular glutathione might be the key positive regulator mediating the difference in proliferative capacity between F and S cell lines. The qRT-PCR assay validated 11 differentially expressed genes related to glutathione metabolism. Exogenous glutathione and its synthase inhibitor L-buthionine-sulfoximine treatment assay demonstrated the positive role of glutathione in the proliferation of Korean pine embryogenic cells.

Annotations

Biosynthesis

Cell lines

Cell proliferation

Conifers

Embryogenesis

Embryos

Genes

Glutathione

Metabolism

Metabolites

Metabolomics

Pine

Regulatory mechanisms (biology)

Signal transduction

Somatic embryogenesis

Details

Title: Subtitle: Glutathione Plays a Positive Role in the Proliferation of Pinus koraiensis Embryogenic Cells
Creators: Fang Gao - Northeast Forestry University
Chunxue Peng
Yue Zhang
Hao Wang
Hailong Shen
Ling Yang
Resource Type: Journal article
Publication Details: International journal of molecular sciences, Vol.23(23), p.14679
DOI: 10.3390/ijms232314679
PMID: 36499020
PMCID: PMC9736457
NLM abbreviation: Int J Mol Sci
ISSN: 1661-6596
eISSN: 1422-0067
Publisher: MDPI AG
Grant note: name: the Innovation Project of State Key Laboratory of Tree Genetics and Breeding, award: 2021B01; name: the National Key R&D Program of China, award: 2017YFD0600600
Language: English
Date published: 12/01/2022
Academic Unit: Molecular Physiology and Biophysics; Anatomy and Cell Biology; Fraternal Order of Eagles Diabetes Research Center
Record Identifier: 9984321947902771

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