Glutathione peroxidase-1 inhibits UVA-induced AP-2α expression in human keratinocytes

Lei Yu; Sujatha Venkataraman; Mitchell C Coleman; Douglas R Spitz; Philip W Wertz; Frederick E Domann

doi:10.1016/j.bbrc.2006.10.171

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Glutathione peroxidase-1 inhibits UVA-induced AP-2α expression in human keratinocytes

Journal article

Peer reviewed

Glutathione peroxidase-1 inhibits UVA-induced AP-2α expression in human keratinocytes

Lei Yu, Sujatha Venkataraman, Mitchell C Coleman, Douglas R Spitz, Philip W Wertz and Frederick E Domann

Biochemical and biophysical research communications, Vol.351(4), pp.1066-1071

2006

DOI: 10.1016/j.bbrc.2006.10.171

PMCID: PMC1913288

PMID: 17097614

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Abstract

In this study, we found a role for H 2O 2 in UVA-induced AP-2α expression in the HaCaT human keratinocyte cell line. UVA irradiation not only increased AP-2α, but also caused accumulation of H 2O 2 in the cell culture media, and H 2O 2 by itself could induce the expression of AP-2α. By catalyzing the removal of H 2O 2 from cells through over-expression of GPx-1, induction of AP-2α expression by UVA was abolished. Induction of transcription factor AP-2α by UVA had been previously shown to be mediated through the second messenger ceramide. We found that not only UVA irradiation, but also H 2O 2 by itself caused increases of ceramide in HaCaT cells, and C2-ceramide added to cells induced the AP-2α signaling pathway. Finally, forced expression of GPx-1 eliminated UVA-induced ceramide accumulation as well as AP-2α expression. Taken together, these findings suggest that GPx-1 inhibits UVA-induced AP-2α expression by suppressing the accumulation of H 2O 2.

Signal Transduction

Oxidative Stress

Reactive oxygen species

Skin

Antioxidant enzyme

Details

Title: Subtitle: Glutathione peroxidase-1 inhibits UVA-induced AP-2α expression in human keratinocytes
Creators: Lei Yu - Free Radical and Radiation Biology Program, Department of Radiation Oncology, Carver College of Medicine, and Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, IA 52242, USA
Sujatha Venkataraman - Free Radical and Radiation Biology Program, Department of Radiation Oncology, Carver College of Medicine, and Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, IA 52242, USA
Mitchell C Coleman - Free Radical and Radiation Biology Program, Department of Radiation Oncology, Carver College of Medicine, and Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, IA 52242, USA
Douglas R Spitz - Free Radical and Radiation Biology Program, Department of Radiation Oncology, Carver College of Medicine, and Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, IA 52242, USA
Philip W Wertz - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA
Frederick E Domann - Free Radical and Radiation Biology Program, Department of Radiation Oncology, Carver College of Medicine, and Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Biochemical and biophysical research communications, Vol.351(4), pp.1066-1071
DOI: 10.1016/j.bbrc.2006.10.171
PMID: 17097614
PMCID: PMC1913288
NLM abbreviation: Biochem Biophys Res Commun
ISSN: 0006-291X
eISSN: 1090-2104
Publisher: Elsevier BV
Language: English
Date published: 2006
Academic Unit: Oral Pathology, Radiology and Medicine; Pathology; Orthopedics and Rehabilitation; Surgery; Radiation Oncology
Record Identifier: 9984040280202771

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