Journal article
Glycaemic status affects the subgingival microbiome of diabetic patients
Journal of clinical periodontology, Vol.45(8), pp.932-940
08/2018
DOI: 10.1111/jcpe.12908
PMID: 29742293
Abstract
Periodontitis is correlated with type 2 diabetes mellitus (T2DM), but little is known about glycaemic status effect on subgingival microbiota associated with periodontitis. This study evaluated if periodontal microbiome of T2DM patients is affected by glycaemic status.
Twenty-one T2DM non-smoking patients with chronic periodontitis and body mass index ≤40 kg/m
were allocated into two groups according to systemic glycaemic status: inadequate (DMI- HbA1c ≥ 8%) and adequate (DMA- HbA1c <7.8%). Subgingival biofilm was collected from sites with moderate (PD = 4-6 mm) and severe disease (PD ≥ 7 mm) in two quadrants. The V5-V6 hypervariable region of the 16SrRNA was sequenced using the GS-FLX-454 Titanium platform. Sequences were compared with HOMD database using QIIME and PhyloToAST pipelines. Statistical comparisons were made using two-sample t-tests.
DMA microbiome presented higher diversity than DMI. Inadequate glycaemic control favoured fermenting species, especially those associated with propionate/succinate production, whereas those forming butyrate/pyruvate was decreased in DMI. Higher abundances of anginosus group and Streptococcus agalactiae in DMI may indicate that subgingival sites can be reservoir of potentially invasive pathogens. Altered subgingival microbiome in DMI may represent an additional challenge in the periodontal treatment of these patients and in the prevention of more invasive infections.
Glycaemic status in T2DM patients seems to modulate subgingival biofilm composition.
Details
- Title: Subtitle
- Glycaemic status affects the subgingival microbiome of diabetic patients
- Creators
- Priscila L Longo - Universidade de São PauloShareef Dabdoub - The Ohio State UniversityPurnima Kumar - The Ohio State UniversityHilana P C Artese - Universidade de São PauloSergio A Dib - Universidade Federal de São PauloGiuseppe A Romito - Universidade de São PauloMarcia Pinto Alves Mayer - Universidade de São Paulo
- Resource Type
- Journal article
- Publication Details
- Journal of clinical periodontology, Vol.45(8), pp.932-940
- DOI
- 10.1111/jcpe.12908
- PMID
- 29742293
- NLM abbreviation
- J Clin Periodontol
- ISSN
- 0303-6979
- eISSN
- 1600-051X
- Grant note
- T32 DE014320 / NIDCR NIH HHS
- Language
- English
- Date published
- 08/2018
- Academic Unit
- Dental Research; Periodontics
- Record Identifier
- 9984367757602771
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