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Glycerol Monolaurate (GML) and a Nonaqueous Five-Percent GML Gel Kill Bacillus and Clostridium Spores
Journal article   Open access   Peer reviewed

Glycerol Monolaurate (GML) and a Nonaqueous Five-Percent GML Gel Kill Bacillus and Clostridium Spores

Patrick M Schlievert, Samuel H Kilgore, Gabriela M Kaus, Theresa D Ho and Craig D Ellermeier
mSphere, Vol.3(6), p.e00597-18
11/21/2018
DOI: 10.1128/mSphereDirect.00597-18
PMCID: PMC6249644
PMID: 30463926
url
https://doi.org/10.1128/mSphereDirect.00597-18View
Published (Version of record) Open Access

Abstract

Glycerol monolaurate is a broadly antimicrobial fatty acid monoester, killing bacteria, fungi, and enveloped viruses. The compound kills stationary-phase cultures of , suggesting that the molecule may kill spores. In this study, we examined the ability of glycerol monolaurate alone or solubilized in a nonaqueous gel to kill vegetative cells and spores of aerobic , , and and anaerobic and ( ) Glycerol monolaurate alone was bactericidal for all five organisms tested. Glycerol monolaurate alone was effective in killing spores. When solubilized in a nonaqueous gel, the glycerol monolaurate gel was bactericidal for all spores tested. The data suggest that glycerol monolaurate nonaqueous gel could be effective in decontaminating environmental and body surfaces, such as skin. and spores are known to be highly resistant to killing, persisting on environmental and human body surfaces for long periods of time. In favorable environments, these spores may germinate and cause human diseases. It is thus important to identify agents that can be used on both environmental and human skin and mucosal surfaces and that are effective in killing spores. We previously showed that the fatty acid monoester glycerol monolaurate (GML) kills stationary-phase cultures of Since such cultures are likely to contain spores, it is possible that GML and a human-use-approved GML nonaqueous gel would kill and spores. The significance of our studies is that we have identified GML, and, to a greater extent, GML solubilized in a nonaqueous gel, as effective in killing spores from both bacterial genera.
Clostridium endospores glycerol monolaurate Bacillus

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