Glycerol Monolaurate (GML) inhibits human T cell signaling and function by disrupting lipid dynamics

Michael S Zhang; Aline Sandouk; Jon C D Houtman

doi:10.1038/srep30225

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Glycerol Monolaurate (GML) inhibits human T cell signaling and function by disrupting lipid dynamics

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Glycerol Monolaurate (GML) inhibits human T cell signaling and function by disrupting lipid dynamics

Michael S Zhang, Aline Sandouk and Jon C D Houtman

Scientific reports, Vol.6(1), pp.30225-30225

07/26/2016

DOI: 10.1038/srep30225

PMCID: PMC4960522

PMID: 27456316

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Abstract

Glycerol Monolaurate (GML) is a naturally occurring fatty acid widely utilized in food, cosmetics, and homeopathic supplements. GML is a potent antimicrobial agent that targets a range of bacteria, fungi, and enveloped viruses but select findings suggest that GML also has immunomodulatory functions. In this study, we have mechanistically examined if GML affects the signaling and functional output of human primary T cells. We found that GML potently altered order and disorder dynamics in the plasma membrane that resulted in reduced formation of LAT, PLC-γ, and AKT microclusters. Altered membrane events induced selective inhibition of TCR-induced phosphorylation of regulatory P85 subunit of PI3K and AKT as well as abrogated calcium influx. Ultimately, GML treatment potently reduced TCR-induced production of IL-2, IFN-γ, TNF-α, and IL-10. Our data reveal that the widely used anti-microbial agent GML also alters the lipid dynamics of human T cells, leading to their defective signaling and function.

Details

Title: Subtitle: Glycerol Monolaurate (GML) inhibits human T cell signaling and function by disrupting lipid dynamics
Creators: Michael S Zhang - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, United States
Aline Sandouk - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, United States
Jon C D Houtman - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, United States
Resource Type: Journal article
Publication Details: Scientific reports, Vol.6(1), pp.30225-30225
DOI: 10.1038/srep30225
PMID: 27456316
PMCID: PMC4960522
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Grant note: T32 AI007343 / NIAID NIH HHS T32 GM007337 / NIGMS NIH HHS R01 CA136729 / NCI NIH HHS
Language: English
Date published: 07/26/2016
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984094333202771

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