Glycogen depletion can increase the specificity of mucin detection in airway tissues

David K Meyerholz; Amanda P Beck; J Adam Goeken; Mariah R Leidinger; Georgina K Ofori-Amanfo; Hannah C Brown; Thomas R Businga; David A Stoltz; Leah R Reznikov; Heather A Flaherty

doi:10.1186/s13104-018-3855-y

Back

Glycogen depletion can increase the specificity of mucin detection in airway tissues

Journal article

Open access

Peer reviewed

Glycogen depletion can increase the specificity of mucin detection in airway tissues

David K Meyerholz, Amanda P Beck, J Adam Goeken, Mariah R Leidinger, Georgina K Ofori-Amanfo, Hannah C Brown, Thomas R Businga, David A Stoltz, Leah R Reznikov and Heather A Flaherty

BMC research notes, Vol.11(1), pp.763-763

10/25/2018

DOI: 10.1186/s13104-018-3855-y

PMCID: PMC6203197

PMID: 30359291

Files and links (1)

url

https://doi.org/10.1186/s13104-018-3855-yView

Published (Version of record) Open Access

Abstract

Mucin is an important parameter for detection and assessment in studies of airway disease including asthma and cystic fibrosis. Histochemical techniques are often used to evaluate mucin in tissues sections. Periodic acid Schiff (PAS) is a common technique to detect neutral mucins in tissue, but this technique also detects other tissue components including cellular glycogen. We tested whether depletion of glycogen, a common cellular constituent, could impact the detection of mucin in the surface epithelium of the trachea. Normal tissues stained by PAS had significantly more staining than serial sections of glycogen-depleted tissue with PAS staining (i.e. dPAS technique) based on both quantitative analysis and semiquantitative scores. Most of the excess stain by the PAS technique was detected in ciliated cells adjacent to goblet cells. We also compared normal tissues using the Alcian blue technique, which does not have reported glycogen staining, with the dPAS technique. These groups had similar amounts of staining consistent with a high degree of mucin specificity. Our results suggest that when using PAS techniques to stain airways, the dPAS approach is preferred as it enhances the specificity for airway mucin.

Periodic acid Schiff (PAS)

Specificity

Diastase-periodic acid Schiff (dPAS)

Mucin

Glycogen

Staining

Nonspecific staining

Scoring

Mucus

Alcian blue

Details

Title: Subtitle: Glycogen depletion can increase the specificity of mucin detection in airway tissues
Creators: David K Meyerholz - University of Iowa
Amanda P Beck - Albert Einstein College of Medicine
J Adam Goeken - University of Iowa
Mariah R Leidinger - University of Iowa
Georgina K Ofori-Amanfo - University of Iowa
Hannah C Brown - University of Iowa
Thomas R Businga - University of Iowa
David A Stoltz - University of Iowa
Leah R Reznikov - University of Florida
Heather A Flaherty - Iowa State University
Resource Type: Journal article
Publication Details: BMC research notes, Vol.11(1), pp.763-763
DOI: 10.1186/s13104-018-3855-y
PMID: 30359291
PMCID: PMC6203197
NLM abbreviation: BMC Res Notes
ISSN: 1756-0500
eISSN: 1756-0500
Grant note: P30 ES005605 / NIEHS NIH HHS HL51670 / National Heart, Lung, and Blood Institute HL136813 / National Heart, Lung, and Blood Institute HL119560 / National Heart, Lung, and Blood Institute R00 HL119560 / NHLBI NIH HHS HL091842 / National Heart, Lung, and Blood Institute DK54759 / National Institute of Diabetes and Digestive and Kidney Diseases P01 HL091842 / NHLBI NIH HHS UIOWA / Cystic Fibrosis Foundation R01 HL136813 / NHLBI NIH HHS P01 HL051670 / NHLBI NIH HHS P30 DK054759 / NIDDK NIH HHS K99 HL119560 / NHLBI NIH HHS OT2 OD023854 / NIH HHS
Language: English
Date published: 10/25/2018
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Pathology; Internal Medicine
Record Identifier: 9984186405902771

Metrics

13 Record Views