Glycoprotein 46 mRNA abundance is post-transcriptionally regulated during development of Leishmania chagasi promastigotes to an infectious form

Jeffrey K Beetham; Karen S Myung; James J McCoy; Mary E Wilson; John E Donelson

doi:10.1074/jbc.272.28.17360

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Glycoprotein 46 mRNA abundance is post-transcriptionally regulated during development of Leishmania chagasi promastigotes to an infectious form

Journal article

Open access

Peer reviewed

Glycoprotein 46 mRNA abundance is post-transcriptionally regulated during development of Leishmania chagasi promastigotes to an infectious form

Jeffrey K Beetham, Karen S Myung, James J McCoy, Mary E Wilson and John E Donelson

The Journal of biological chemistry, Vol.272(28), pp.17360-17366

07/11/1997

DOI: 10.1074/jbc.272.28.17360

PMID: 9211875

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Abstract

GP46 is an abundant glycoprotein of 46 kDa on the surface of the promastigote form of most Leishmania species. We show that the steady state level of GP46 mRNA increases >>30-fold as Leishmania chagasi promastigotes develop in vitro from a less infectious form during logarithmic growth to a highly infectious form in the stationary phase of cultivation. Nuclear run-on experiments demonstrate that this increase in GP46 mRNA abundance is regulated post-transcriptionally. Plasmids containing the 3'-untranslated regions (UTRs) and downstream intergenic regions (IRs) of two different GP46 genes fused immediately downstream of the beta-galactosidase coding region were transfected into L. chagasi, and beta-galactosidase activity and mRNA levels were examined. The presence of the 3'-UTR + IR of one GP46 gene (gp46A) resulted in a steady increase in beta-galactosidase activity and mRNA level as the transfected promastigotes developed from logarithmic to stationary phase. This differential effect parallels that of the 3'-UTRs + IRs of a family of genes for an unrelated Leishmania surface glycoprotein, GP63. Thus, post-transcriptional regulation of the genes for two different surface glycoproteins of Leishmania occurs via a similar mechanism.

Transfection

Amino Acid Sequence

Membrane Glycoproteins - metabolism

RNA Processing, Post-Transcriptional

Molecular Sequence Data

Chromosome Mapping

Protozoan Proteins - genetics

RNA, Messenger - metabolism

DNA, Complementary - isolation & purification

Membrane Glycoproteins - genetics

Sequence Alignment

Animals

Protozoan Proteins - metabolism

Base Sequence

Transcription, Genetic

Leishmania - pathogenicity

beta-Galactosidase - genetics

Leishmania - growth & development

Genes, Reporter

Details

Title: Subtitle: Glycoprotein 46 mRNA abundance is post-transcriptionally regulated during development of Leishmania chagasi promastigotes to an infectious form
Creators: Jeffrey K Beetham - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
Karen S Myung
James J McCoy
Mary E Wilson
John E Donelson
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.272(28), pp.17360-17366
DOI: 10.1074/jbc.272.28.17360
PMID: 9211875
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: HL07121 / NHLBI NIH HHS AI32135 / NIAID NIH HHS
Language: English
Date published: 07/11/1997
Academic Unit: Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984002394602771

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