Glycoproteomic study reveals altered plasma proteins associated with HIV elite suppressors

Weiming Yang; Oliver Laeyendecker; Sarah K Wendel; Bai Zhang; Shisheng Sun; Jian-Ying Zhou; Minghui Ao; Richard D Moore; J Brooks Jackson; Hui Zhang

doi:10.7150/thno.9510

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Glycoproteomic study reveals altered plasma proteins associated with HIV elite suppressors

Journal article

Open access

Peer reviewed

Glycoproteomic study reveals altered plasma proteins associated with HIV elite suppressors

Weiming Yang, Oliver Laeyendecker, Sarah K Wendel, Bai Zhang, Shisheng Sun, Jian-Ying Zhou, Minghui Ao, Richard D Moore, J Brooks Jackson and Hui Zhang

Theranostics, Vol.4(12), pp.1153-1163

2014

DOI: 10.7150/thno.9510

PMCID: PMC4183994

PMID: 25285165

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Abstract

HIV elite suppressors (ES) or controllers are individuals achieving control of viremia by their natural immunological mechanisms without highly active antiretroviral therapy (HAART). Study of the mechanisms responsible for the immunological suppression of viremia in ES may lead to the detection of individuals with ES and the effective control of HIV infection. We hypothesize that plasma glycoproteins play essential roles in the immune system of ES since plasma proteins are critical and highly relevant in anti-viral immunity and most plasma proteins are glycoproteins. To examine glycoproteins associated with ES, plasma samples from ES individuals (n=20), and from individuals on HAART (n=20), with AIDS (n=20), and no HIV infection (n=10) were analyzed by quantitative glycoproteomics. We found that a number of glycoproteins changed between ES versus HAART, AIDS and HIV- individuals. In sharp contrast, the level of plasma glycoproteins in the HAART cohort showed fewer changes compared with AIDS and HIV- individuals. These results showed that although both ES and HAART effectively suppress viremia, ES appeared to profoundly affect immunologically relevant glycoproteins in plasma as consequence of or support for anti-viral immunity. Bioinformatic analysis revealed that altered proteins in ES plasma were mainly associated with inflammation. This analysis suggests that overlapping, while distinguishable, glycoprotein profiles for inflammation and immune activation appeared to be present between ES and non-ES (HAART+AIDS) cohorts, indicating different triggers for inflammation and immune activation between natural and treatment-related viral suppression.

Mass Spectrometry

HIV Infections - blood

Glycomics

HIV Infections - virology

Humans

Blood Proteins - immunology

Male

Glycoproteins - blood

Blood Proteins - chemistry

Antiretroviral Therapy, Highly Active

HIV Infections - immunology

Glycoproteins - immunology

HIV-1 - immunology

Adult

Anti-HIV Agents - therapeutic use

Female

HIV Infections - drug therapy

Glycoproteins - chemistry

Cohort Studies

Details

Title: Subtitle: Glycoproteomic study reveals altered plasma proteins associated with HIV elite suppressors
Creators: Weiming Yang - 1. Department of Pathology, , Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Oliver Laeyendecker - 2. Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; ; 3. Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Baltimore, Maryland, USA
Sarah K Wendel - 3. Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Baltimore, Maryland, USA
Bai Zhang - 1. Department of Pathology, , Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Shisheng Sun - 1. Department of Pathology, , Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Jian-Ying Zhou - 1. Department of Pathology, , Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Minghui Ao - 1. Department of Pathology, , Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Richard D Moore - 2. Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
J Brooks Jackson - 1. Department of Pathology, , Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Hui Zhang - Johns Hopkins University
Resource Type: Journal article
Publication Details: Theranostics, Vol.4(12), pp.1153-1163
DOI: 10.7150/thno.9510
PMID: 25285165
PMCID: PMC4183994
NLM abbreviation: Theranostics
ISSN: 1838-7640
eISSN: 1838-7640
Publisher: Australia
Grant note: U01 CA152813 / NCI NIH HHS 1U01 AI69482-01 / NIAID NIH HHS U24 CA160036 / NCI NIH HHS P01 HL107153 / NHLBI NIH HHS U24CA115102 / NCI NIH HHS P01HL107153 / NHLBI NIH HHS U01 AI069482 / NIAID NIH HHS U01 DA036935 / NIDA NIH HHS UM1 AI068613 / NIAID NIH HHS R01CA112314 / NCI NIH HHS N01-HV-00240 / NHLBI NIH HHS U24 CA115102 / NCI NIH HHS U24CA160036 / NCI NIH HHS R01 CA112314 / NCI NIH HHS
Language: English
Date published: 2014
Academic Unit: Pathology; VPMA - Administration
Record Identifier: 9984047875702771

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