Glyoxal induced structural transition of buffalo kidney cystatin to molten globule and aggregates: Anti-fibrillation potency of quinic acid

Anas Shamsi; Azaj Ahmed; Bilqees Bano

doi:10.1002/iub.1471

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Glyoxal induced structural transition of buffalo kidney cystatin to molten globule and aggregates: Anti-fibrillation potency of quinic acid

Journal article

Peer reviewed

Glyoxal induced structural transition of buffalo kidney cystatin to molten globule and aggregates: Anti-fibrillation potency of quinic acid

Anas Shamsi, Azaj Ahmed and Bilqees Bano

IUBMB life, Vol.68(2), pp.156-166

02/2016

DOI: 10.1002/iub.1471

PMID: 26748578

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Abstract

In our study buffalo kidney cystatin (BKC) is transformed from native conformation to amyloid fibrils when incubated with 20 mM glyoxal for a prolonged time period. These amyloid fibrils are at the heart of a number of pathological disorders. In the presence of 10 mM glyoxal, BKC retained native-like secondary structure, decreased intrinsic and increased ANS fluorescence was observed, characteristics of molten globule state (MG), thus suggesting the occurrence of MG state at this concentration. At 20 mM glyoxal, BKC aggregates were characterized by a further decrease in ANS fluorescence attributable to internalization of hydrophobic clusters owing to protein-protein interaction. Circular dichroism and FTIR spectroscopy further revealed the existence of β sheet structure and there was an increase in Thioflavin T fluorescence, Rayleigh light scattering, turbidity as well as red shift in congo red absorbance thus confirming the existence of aggregates. We have also studied the anti-aggregation effect of polyol, quinic acid making use of various above mentioned biophysical assays. Our proposed work strongly favors the formation of BKC aggregates in the presence of glyoxal, which is present in higher amounts in pathological conditions owing to defective glycolysis pathway and also use of polyol as an antifibrillating agent.

Amyloid - drug effects

Amyloid - metabolism

Animals

Buffaloes

Circular Dichroism

Cystatins - metabolism

Glyoxal - metabolism

Kidney - drug effects

Kidney - metabolism

Kidney - pathology

Protein Aggregation, Pathological - drug therapy

Protein Aggregation, Pathological - metabolism

Protein Aggregation, Pathological - pathology

Protein Structure, Secondary - drug effects

Quinic Acid - administration & dosage

Spectroscopy, Fourier Transform Infrared

Details

Title: Subtitle: Glyoxal induced structural transition of buffalo kidney cystatin to molten globule and aggregates: Anti-fibrillation potency of quinic acid
Creators: Anas Shamsi - Aligarh Muslim University
Azaj Ahmed - Aligarh Muslim University
Bilqees Bano - Aligarh Muslim University
Resource Type: Journal article
Publication Details: IUBMB life, Vol.68(2), pp.156-166
DOI: 10.1002/iub.1471
PMID: 26748578
ISSN: 1521-6543
eISSN: 1521-6551
Language: English
Date published: 02/2016
Academic Unit: Internal Medicine
Record Identifier: 9985178663002771

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