Journal article
Glypican 4 regulates planar cell polarity of endoderm cells by controlling the localization of Cadherin 2
Development (Cambridge), Vol.148(14), dev199421
07/01/2021
DOI: 10.1242/dev.199421
PMCID: PMC8313861
PMID: 34131730
Abstract
Noncanonical Wnt/planar cell polarity (Wnt/PCP) signaling has been implicated in endoderm morphogenesis. However, the underlying cellular and molecular mechanisms of this process are unclear. We found that, during convergence and extension (C&E) in zebrafish, gut endodermal cells are polarized mediolaterally, with GFP-Vangl2 enriched at the anterior edges. Endoderm cell polarity is lost and intercalation is impaired in the absence of glypican 4 (gpc4), a heparan-sulfate proteoglycan that promotes Wnt/PCP signaling, suggesting that this signaling is required for endodermal cell polarity. Live imaging revealed that endoderm C&E is accomplished by polarized cell protrusions and junction remodeling, which are impaired in gpc4-deficient endodermal cells. Furthermore, in the absence of gpc4, Cadherin 2 expression on the endodermal cell surface is increased as a result of impaired Rab5c-mediated endocytosis, which partially accounts for the endodermal defects in these mutants. These findings indicate that Gpc4 regulates endodermal planar cell polarity during endoderm C&E by influencing the localization of Cadherin 2. Thus, our study uncovers a new mechanism by which Gpc4 regulates planar cell polarity and reveals the role of Wnt/PCP signaling in endoderm morphogenesis.
Details
- Title: Subtitle
- Glypican 4 regulates planar cell polarity of endoderm cells by controlling the localization of Cadherin 2
- Creators
- Anurag Kakkerla Balaraju - Roy J. and Lucille A. Carver College of MedicineBo Hu - Roy J. and Lucille A. Carver College of MedicineJuan J. Rodriguez - Roy J. and Lucille A. Carver College of MedicineMatthew Murry - Roy J. and Lucille A. Carver College of MedicineFang Lin - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Development (Cambridge), Vol.148(14), dev199421
- DOI
- 10.1242/dev.199421
- PMID
- 34131730
- PMCID
- PMC8313861
- NLM abbreviation
- Development
- ISSN
- 0950-1991
- eISSN
- 1477-9129
- Publisher
- Company Biologists Ltd
- Number of pages
- 15
- Grant note
- 1R56DK123610-01 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 07/01/2021
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984288758002771
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