Journal article
Gonococcal lipooligosaccharide is a ligand for the asialoglycoprotein receptor on human sperm
Molecular microbiology, Vol.36(5), pp.1059-1070
06/2000
DOI: 10.1046/j.1365-2958.2000.01938.x
PMID: 10844691
Abstract
In the present study, we show that Neisseria gonorrhoeae lipooligosaccharide (LOS) can bind to the asialoglycoprotein receptor (ASGP-R) on human sperm. This work demonstrates the presence of ASGP-R on human sperm. Binding of purified ASGP-R ligand decreased in the presence of gonococci. Binding of purified iodinated gonococcal LOS identified a protein of molecular weight corresponding to that of human ASGP-R. The presence of excess unlabelled LOS blocked binding of iodinated gonococcal LOS. Binding of wild-type gonococcal LOS to sperm was higher than that of mutant LOS lacking the galactose ligand for ASGP-R. These data suggest that the ASGP-R on human sperm cells recognizes and binds wild-type gonococcal LOS. This interaction may contribute to the transmission of gonorrhea from infected males to their sexual partners.
Details
- Title: Subtitle
- Gonococcal lipooligosaccharide is a ligand for the asialoglycoprotein receptor on human sperm
- Creators
- H A Harvey - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. Pediatric Infectious Disease Unit, Soroka University Medical Center, P.O.B. 151, Beer Sheva, IsraelN PoratC A CampbellM JenningsB W GibsonN J PhillipsM A ApicellaM S Blake
- Resource Type
- Journal article
- Publication Details
- Molecular microbiology, Vol.36(5), pp.1059-1070
- DOI
- 10.1046/j.1365-2958.2000.01938.x
- PMID
- 10844691
- NLM abbreviation
- Mol Microbiol
- ISSN
- 0950-382X
- eISSN
- 1365-2958
- Grant note
- AI38515 / NIAID NIH HHS AI43924 / NIAID NIH HHS AI18384 / NIAID NIH HHS
- Language
- English
- Date published
- 06/2000
- Academic Unit
- Microbiology and Immunology; Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984094705002771
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