Gq/11-Dependent Changes in the Murine Ovarian Transcriptome at the End of Gestation

Courtney Waite; Rachel Mejia; Mario Ascoli

doi:10.1095/biolreprod.115.136952

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Gq/11-Dependent Changes in the Murine Ovarian Transcriptome at the End of Gestation

Journal article

Open access

Peer reviewed

Gq/11-Dependent Changes in the Murine Ovarian Transcriptome at the End of Gestation

Courtney Waite, Rachel Mejia and Mario Ascoli

Biology of reproduction, Vol.94(3), pp.62-62

03/2016

DOI: 10.1095/biolreprod.115.136952

PMCID: PMC4829089

PMID: 26843449

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https://doi.org/10.1095/biolreprod.115.136952View

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Abstract

Parturition in rodents is highly dependent on the engagement of the luteal prostaglandin F2 alpha receptor, which, through activation of the Gq/11 family of G proteins, increases the expression of Akr1c18, leading to an increase in progesterone catabolism. To further understand the involvement of Gq/11 on luteolysis and parturition, we used microarray analysis to compare the ovarian transcriptome of mice with a granulosa/luteal cell-specific deletion of Galphaq/11 with their control littermates on Day 18 of pregnancy, when mice from both genotypes are pregnant, and on Day 22, when mice with a granulosa/luteal cell-specific deletion of Galphaq/11 are still pregnant but their control littermates are 1-2 days postpartum. Ovarian genes up-regulated at the end of gestation in a Galphaq/11 -dependent fashion include genes involved in focal adhesion and extracellular matrix interactions. Genes down-regulated at the end of gestation in a Galphaq/11-dependent manner include Serpina6 (which encodes corticosteroid-binding globulin); Enpp2 (which encodes autotaxin, the enzyme responsible for the synthesis of lysophosphatidic acid); genes involved in protein processing and export; reproductive genes, such as Lhcgr; the three genes needed to convert progesterone to estradiol (Cyp17a1, Hsd17b7, and Cyp19a1); and Inha. Activation of ovarian Gq/11 by engagement of the prostaglandin F2 alpha receptor on Day 18 of pregnancy recapitulated the regulation of many but not all of these genes. Thus, although the ovarian transcriptome at the end of gestation is highly dependent on the activation of Gq/11, not all of these changes are dependent on the actions of prostaglandin F2 alpha.

GTP-Binding Protein alpha Subunits, Gq-G11 - genetics

Luteal Cells

Up-Regulation

Oligonucleotide Array Sequence Analysis

Down-Regulation

Transcriptome

Gene Expression Regulation - physiology

Genotype

Mice, Knockout

Pregnancy

Animals

Cloprostenol - pharmacology

Polymerase Chain Reaction

Female

Mice

GTP-Binding Protein alpha Subunits, Gq-G11 - metabolism

Details

Title: Subtitle: Gq/11-Dependent Changes in the Murine Ovarian Transcriptome at the End of Gestation
Creators: Courtney Waite - Department of Pharmacology, Carver College of Medicine, University of Iowa, Iowa City, Iowa
Rachel Mejia - Department of Obstetrics and Gynecology, Carver College of Medicine, University of Iowa, Iowa City, Iowa
Mario Ascoli - Department of Pharmacology, Carver College of Medicine, University of Iowa, Iowa City, Iowa mario-ascoli@uiowa.edu
Resource Type: Journal article
Publication Details: Biology of reproduction, Vol.94(3), pp.62-62
Publisher: United States
DOI: 10.1095/biolreprod.115.136952
PMID: 26843449
PMCID: PMC4829089
ISSN: 0006-3363
eISSN: 1529-7268
Grant note: P50 HD028934 / NICHD NIH HHS K12 HD063117 / NICHD NIH HHS P50-HD28934 / NICHD NIH HHS
Language: English
Date published: 03/2016
Academic Unit: Obstetrics and Gynecology; Neuroscience and Pharmacology
Record Identifier: 9983931813802771

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