Journal article
Gradient polyacrylamide gel electrophoresis for determination of molecular weights of heparin preparations and low-molecular-weight heparin derivatives
Journal of pharmaceutical sciences, Vol.81(8), pp.823-827
08/1992
DOI: 10.1002/jps.2600810821
PMID: 1328601
Abstract
The M(r) values of pharmaceutical heparins and low-molecular-weight (LMW) heparin derivatives were examined as part of a collaborative study to develop methods for their characterization. Standard methods of M(r) determination rely on gel permeation high-performance liquid chromatography (HPLC). We report the use of gradient polyacrylamide gel electrophoresis (PAGE) to determine the M(r) values of pharmaceutical heparins and LMW heparin derivatives. This approach offers certain advantages over the HPLC method. Gradient PAGE analysis was performed in parallel, on multiple samples, with the same standard curve. HPLC was performed serially. Gradient PAGE gave higher resolution than HPLC, and thus, a mixture of easily obtained standards was used in place of individual standards for the construction of a standard curve. Heparin and various LMW heparin samples were analyzed by both gradient PAGE and conventional gel permeation HPLC methods. The number-average M(r), weight-average M(r), and polydispersity were examined by both techniques and found to be similar. This study demonstrates that gradient PAGE analysis is a sensitive method for the determination of the M(r) values of heparin and LMW heparin.
Details
- Title: Subtitle
- Gradient polyacrylamide gel electrophoresis for determination of molecular weights of heparin preparations and low-molecular-weight heparin derivatives
- Creators
- R E Edens - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City 52242A al-HakimJ M WeilerD G RethwischJ FareedR J Linhardt
- Resource Type
- Journal article
- Publication Details
- Journal of pharmaceutical sciences, Vol.81(8), pp.823-827
- DOI
- 10.1002/jps.2600810821
- PMID
- 1328601
- NLM abbreviation
- J Pharm Sci
- ISSN
- 0022-3549
- eISSN
- 1520-6017
- Publisher
- United States
- Grant note
- GM38060 / NIGMS NIH HHS HL29797 / NHLBI NIH HHS AI 22350 / NIAID NIH HHS
- Language
- English
- Date published
- 08/1992
- Academic Unit
- Chemical and Biochemical Engineering; Internal Medicine
- Record Identifier
- 9984003414202771
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