Grain dust-induced airflow obstruction and inflammation of the lower respiratory tract

W D Clapp; S Becker; J Quay; J L Watt; P S Thorne; K L Frees; X Zhang; H S Koren; C R Lux; D A Schwartz

doi:10.1164/ajrccm.150.3.8087327

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Grain dust-induced airflow obstruction and inflammation of the lower respiratory tract

Journal article

Peer reviewed

Grain dust-induced airflow obstruction and inflammation of the lower respiratory tract

W D Clapp, S Becker, J Quay, J L Watt, P S Thorne, K L Frees, X Zhang, H S Koren, C R Lux and D A Schwartz

American journal of respiratory and critical care medicine, Vol.150(3), pp.611-617

09/1994

DOI: 10.1164/ajrccm.150.3.8087327

PMID: 8087327

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Abstract

To investigate the relationship between the physiologic and biologic effects of grain dust inhalation, we exposed 15 nonsmoking, nonasthmatic, nonatopic male grain handlers to buffered saline and aqueous corn dust extract by inhalation challenge in a crossover study. The inhalation challenges to buffered saline and corn dust extract were separated by at least 14 d. Compared with buffered saline, inhalation of corn dust extract resulted in significant airflow obstruction, which was observed within 30 min of exposure and persisted for 5 h. Inhalation of corn dust extract resulted in an acute inflammatory response characterized by higher concentrations of neutrophils (p = 0.001), IL-1 beta (p = 0.001), IL-1RA (p = 0.001), IL-6 (p = 0.001), IL-8 (p = 0.001), and TNF-alpha (p = 0.04) in bronchoalveolar lavage (BAL) fluid. mRNA levels specific for IL-1 beta, IL-1RA, IL-6, and IL-8 from cells present in the BAL fluid were significantly greater after challenge with corn dust extract than after challenge with buffered saline. Importantly, no significant differences were observed in the concentration of lymphocytes or eosinophils in the BAL fluid following inhalation of corn dust extract, and the concentrations of histamine and 15-HETE were similar in BAL fluid after the two challenges. The maximal percentage decrease in FEV1 was significantly associated with the absolute neutrophil concentration in the BAL fluid (p = 0.001), as well as the concentration of TNF-alpha (p = 0.03), IL-1 beta (p = 0.005), IL-1RA (p = 0.001), IL-6 (p = 0.001), and IL-8 (p = 0.001) in the BAL fluid.

Polymerase Chain Reaction

Edible Grain - adverse effects

Cytokines - analysis

Dust - adverse effects

Humans

Middle Aged

Airway Obstruction - diagnosis

RNA, Messenger - analysis

Male

Allergens - administration & dosage

Adult

Agricultural Workers' Diseases - diagnosis

Inflammation - diagnosis

Airway Obstruction - etiology

Airway Obstruction - physiopathology

Respiratory Tract Diseases - diagnosis

Inflammation - etiology

Iowa

Respiratory Tract Diseases - etiology

Adolescent

Agricultural Workers' Diseases - physiopathology

Respiratory Tract Diseases - physiopathology

Bronchoalveolar Lavage Fluid - chemistry

Agricultural Workers' Diseases - etiology

Nebulizers and Vaporizers

Inflammation - physiopathology

Details

Title: Subtitle: Grain dust-induced airflow obstruction and inflammation of the lower respiratory tract
Creators: W D Clapp - Department of Internal Medicine, University of Iowa, Iowa City 52242
S Becker
J Quay
J L Watt
P S Thorne
K L Frees
X Zhang
H S Koren
C R Lux
D A Schwartz
Resource Type: Journal article
Publication Details: American journal of respiratory and critical care medicine, Vol.150(3), pp.611-617
Publisher: United States
DOI: 10.1164/ajrccm.150.3.8087327
PMID: 8087327
ISSN: 1073-449X
eISSN: 1535-4970
Grant note: U07CCU706145 / PHS HHS HL-07638 / NHLBI NIH HHS H00093-01 / PHS HHS
Language: English
Date published: 09/1994
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health; Otolaryngology
Record Identifier: 9983997327802771

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