Journal article
Granuloma T lymphocytes in murine schistosomiasis mansoni have somatostatin receptors and respond to somatostatin with decreased IFN-γ secretion
The Journal of immunology (1950), Vol.149(11), pp.3621-3626
1992
DOI: 10.4049/jimmunol.149.11.3621
PMID: 1358973
Abstract
The granulomas of mice infected with Schistosoma mansoni for 8 wk have macrophages that secrete somatostatin 1-14 (SOM). Within the granuloma, SOM has no known function. To uncover the possible significance of SOM produced within this granulomatous inflammation, we sought SOM receptors on distinct cellular components of the granuloma to identify cells targeted for SOM action. [125I]SOM 1-14 bound to dispersed granuloma inflammatory cells specifically and reversibly. Scatchard analysis suggested one receptor type (kDa 4.28 x 10(-9) M). Octreotide, a stable SOM derivative, displaced radioligand (kDa 1.01 x 10(-10) M), but SOM 1-28, substance P, and vasoactive intestinal peptide did not. The SOM receptor localized exclusively to a subset of granuloma CD4+ T lymphocytes. Using IL-5 and IFN-gamma ELISA, it was shown that granuloma T cells can secrete appreciable IL-5 and IFN-gamma when stimulated with Ag or mitogen. Both SOM and octreotide at concentrations as low as 10(-10) M substantially decreased IFN-gamma secretion from Ag or mitogen-stimulated T cells, but at concentrations as high as 10(-6) did not affect IL-5 production. Octreotide administered to animals in vivo decreased the intensity of the granulomatous response. Thus, some granuloma T cells have SOM 1-14 receptors. SOM 1-14, a product of granuloma macrophages, may participate in regulation of the granulomatous response by modulating the secretion of some lymphokines. Octreotide, a clinically useful SOM analog, mimics the action of SOM on the immune system.
Details
- Title: Subtitle
- Granuloma T lymphocytes in murine schistosomiasis mansoni have somatostatin receptors and respond to somatostatin with decreased IFN-γ secretion
- Creators
- Arthur M Blum - University of IowaAhmed Metwali - University of IowaRanjit C Mathew - Univ. Iowa coll. medicine, dep. internal medcine, div. gastroenterology-hepatology, Iowa city IA 52242, United StatesGeorge Cook - Univ. Iowa coll. medicine, dep. internal medcine, div. gastroenterology-hepatology, Iowa city IA 52242, United StatesDavid Elliott - Univ. Iowa coll. medicine, dep. internal medcine, div. gastroenterology-hepatology, Iowa city IA 52242, United StatesJoel V Weinstock - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.149(11), pp.3621-3626
- Publisher
- American Association of Immunologists
- DOI
- 10.4049/jimmunol.149.11.3621
- PMID
- 1358973
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 1992
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984359666702771
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