Green synthesis of silver nanoparticles using cell extracts of Anabaena doliolum and screening of its antibacterial and antitumor activity

Garvita Singh; Piyoosh K Babele; Shailesh K Shahi; Rajeshwar P Sinha; Madhu B Tyagi; Ashok Kumar

doi:10.4014/jmb.1405.05003

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Green synthesis of silver nanoparticles using cell extracts of Anabaena doliolum and screening of its antibacterial and antitumor activity

Journal article

Peer reviewed

Green synthesis of silver nanoparticles using cell extracts of Anabaena doliolum and screening of its antibacterial and antitumor activity

Garvita Singh, Piyoosh K Babele, Shailesh K Shahi, Rajeshwar P Sinha, Madhu B Tyagi and Ashok Kumar

Journal of microbiology and biotechnology, Vol.24(10), pp.1354-1367

10/2014

DOI: 10.4014/jmb.1405.05003

PMID: 24986675

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Abstract

In the present work, we describe a simple, cheap, and unexplored method for "green" synthesis of silver nanoparticles using cell extracts of the cyanobacterium Anabaena doliolum. An attempt was also made to test the antimicrobial and antitumor activities of the synthesized nanoparticles. Analytical techniques, namely UV-vis spectroscopy, X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), and TEMselected area electron diffraction, were used to elucidate the formation and characterization of silver-cyanobacterial nanoparticles (Ag-CNPs). Results showed that the original color of the cell extract changed from reddish blue to dark brown after addition of silver nitrate solution (1 mM) within 1 h, suggesting the synthesis of Ag-CNPs. That the formation Ag-CNPs indeed occurred was also evident from the spectroscopic analysis of the reaction mixture, wherein a prominent peak at 420 nm was noted. TEM images revealed well-dispersed, spherical Ag- CNPs with a particle size in the range of 10-50 nm. The X-ray diffraction spectrum suggested a crystalline nature of the Ag-CNPs. FTIR analysis indicated the utilization of a hydroxyl (-OH) group in the formation of Ag-CNPs. Ag-CNPs exhibited strong antibacterial activity against three multidrug-resistant bacteria. Additionally, Ag-CNPs strongly affected the survival of Dalton's lymphoma and human carcinoma colo205 cells at a very low concentration. The Ag-CNPs-induced loss of survival of both cell types may be due to the induction of reactive oxygen species generation and DNA fragmentation, resulting in apoptosis. Properties exhibited by the Ag-CNP suggest that it may be used as a potential antibacterial and antitumor agent.

Anabaena - enzymology

Anti-Bacterial Agents - chemistry

Anti-Bacterial Agents - metabolism

Anti-Bacterial Agents - pharmacology

Antineoplastic Agents - chemistry

Antineoplastic Agents - metabolism

Antineoplastic Agents - pharmacology

Cell Line, Tumor

Cell Survival - drug effects

Complex Mixtures - metabolism

Humans

Microbial Sensitivity Tests

Microscopy, Electron, Transmission

Nanoparticles - chemistry

Nanoparticles - metabolism

Nanoparticles - ultrastructure

Silver - chemistry

Silver - metabolism

Silver - pharmacology

Silver Nitrate - metabolism

Spectrophotometry

Spectroscopy, Fourier Transform Infrared

Time Factors

X-Ray Diffraction

Details

Title: Subtitle: Green synthesis of silver nanoparticles using cell extracts of Anabaena doliolum and screening of its antibacterial and antitumor activity
Creators: Garvita Singh - Banaras Hindu University
Piyoosh K Babele
Shailesh K Shahi
Rajeshwar P Sinha
Madhu B Tyagi
Ashok Kumar
Resource Type: Journal article
Publication Details: Journal of microbiology and biotechnology, Vol.24(10), pp.1354-1367
DOI: 10.4014/jmb.1405.05003
PMID: 24986675
NLM abbreviation: J Microbiol Biotechnol
ISSN: 1017-7825
eISSN: 1738-8872
Language: English
Date published: 10/2014
Academic Unit: Pathology; Iowa Neuroscience Institute; Neuroscience and Pharmacology
Record Identifier: 9984186500902771

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