Journal article
Growth and analysis of crystal forms of toxic shock syndrome toxin 1
Proteins, structure, function, and bioinformatics, Vol.17(3), pp.329-334
11/1993
DOI: 10.1002/prot.340170310
PMID: 8272430
Abstract
Native toxic shock syndrom toxin 1 (TSST‐1) purified from Staphylococcus aurius has been crystallized in four different forms. The highest resolution data (2.05 Å) was collected from orthorhombic crystals belonging to the space group C2221. The unit cell dimension are a = 108.7 Å, b = 177.5 Å, c = 97.6 Å. Rotation function analysis of this from indicates that there is trimer of toxin molecules in the asymmetric unit with a local 3‐fold axis parallel to the crystallographic c axis. Crystals of a double mutant of TSST‐1 have been grown which has a single molecule in the asymmetric unit and diffract to 1.9 Å. The space group is P21 with unit cell parameters of a = 44.4 Å, b = 34.0 Å, c = 55.2 Å, β = 93.0°. © 1993 Wiley‐Liss, Inc.
Details
- Title: Subtitle
- Growth and analysis of crystal forms of toxic shock syndrome toxin 1
- Creators
- Cathleen A EarhartG. Sridhar PrasadDebra L MurrayRichard P NovickPatrick M SchlievertDouglas H Ohlendorf
- Resource Type
- Journal article
- Publication Details
- Proteins, structure, function, and bioinformatics, Vol.17(3), pp.329-334
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- DOI
- 10.1002/prot.340170310
- PMID
- 8272430
- ISSN
- 0887-3585
- eISSN
- 1097-0134
- Number of pages
- 6
- Language
- English
- Date published
- 11/1993
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001202002771
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