Growth inhibition of a B cell clone mediated by ligation of IL-4 receptors or membrane IgM

Gail A Bishop; Luis M Ramirez; Gary A Koretzky

doi:10.4049/jimmunol.150.7.2565

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Growth inhibition of a B cell clone mediated by ligation of IL-4 receptors or membrane IgM

Journal article

Peer reviewed

Growth inhibition of a B cell clone mediated by ligation of IL-4 receptors or membrane IgM

Gail A Bishop, Luis M Ramirez and Gary A Koretzky

The Journal of immunology (1950), Vol.150(7), pp.2565-2574

04/01/1993

DOI: 10.4049/jimmunol.150.7.2565

PMID: 8454843

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Abstract

The development of B cell tolerance is believed to involve negative signaling to the B cell derived from the binding of Ag to the B cell surface Ig (sIg). B cell clones that receive negative signals via sIg may provide useful models for studying the mechanisms of negative signaling. We have recently identified a previously undescribed mouse B cell clone, CHB3, which receives growth-inhibitory signals through the binding of IL-4 to its IL-4R or through ligation of its sIgM, but not its sIgD, molecules. Data presented here demonstrate that the negative signal delivered by sIgM, but not that delivered by IL-4R, requires protein kinase C activation and elevated intracellular Ca2+, and is associated with the tyrosine phosphorylation of a number of proteins. Thus, the IL-4R signaling pathway appears to be divergent from the sIgM-mediated pathway. However, growth inhibition mediated via both sIgM and IL-4R can be partially counteracted by a signal delivered through the MHC class II molecule.

Phosphorylation

Signal Transduction

Immunoglobulin M - physiology

Calcium - metabolism

Protein Kinase C - antagonists & inhibitors

Receptors, Mitogen - physiology

Interleukin-4 - pharmacology

Tyrosine - metabolism

Animals

Clone Cells - pathology

Lymphoma, B-Cell - pathology

Growth Inhibitors - physiology

Receptors, Antigen, B-Cell - physiology

Receptors, Interleukin-4

Mice

B-Lymphocytes - pathology

Histocompatibility Antigens Class II - physiology

Details

Title: Subtitle: Growth inhibition of a B cell clone mediated by ligation of IL-4 receptors or membrane IgM
Creators: Gail A Bishop - Department of Microbiology, University of Iowa, Iowa City 52242
Luis M Ramirez
Gary A Koretzky
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.150(7), pp.2565-2574
DOI: 10.4049/jimmunol.150.7.2565
PMID: 8454843
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: AI28847 / NIAID NIH HHS
Language: English
Date published: 04/01/1993
Academic Unit: Microbiology and Immunology; President
Record Identifier: 9984002380302771

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