Journal article
Growth of Cell Colonies in Soft Agar From Biopsies of Different Human Solid Tumors
Cancer research (Chicago, Ill.), Vol.40(11), pp.4151-4158
01/01/1980
PMID: 7471056
Abstract
Colony growth in soft agar of cells disaggregated from 87 solid tumor specimens was evaluated. Tumors were disaggregated by an enzymatic method consisting of microtome slicing of tissues and incubation at 37 degree for 2 hr in Roswell Park Memorial Institute Tissue Culture Medium 1640 containing 10% fetal bovine serum. 0.8% collagenase II, and 0.002% DNase I. Tumor cells were cultured in a two-layer agar system (0.5% agar feeder, 0.3% agar plating layer) in enriched medium with or without addition of different factors.
Details
- Title: Subtitle
- Growth of Cell Colonies in Soft Agar From Biopsies of Different Human Solid Tumors
- Creators
- Z PavelicH SlocumY RustumP CreavenN NowakC Karakousis
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.40(11), pp.4151-4158
- PMID
- 7471056
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 01/01/1980
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359850402771
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