Journal article
Growth of human urological tumors on extracellular matrix as a model for the in vitro cultivation of primary human tumor explants
Cancer research (Chicago, Ill.), Vol.46(7), pp.3653-3662
1986
PMID: 3708595
Abstract
The goal of this study was to establish an optimal in vitro growth assay system for human urological tumor explants. Bovine corneal endotelial cell extracellular matrix (ECM) coated dishes were evaluated as a growth substrate for tumor cultures. Growth success for different urological carcinomas (prostatic, bladder, kidney, and testicular) was compared after seeding fresh surgical explants onto bovine corneal endothelial cell ECM and plastic culture flasks. Tumor samples were disaggregated enzymatically, and 1 X 10(4) cells were seeded onto the different substrates using RPMI 1640 medium containing 10% fetal calf serum and/or different growth factors, nutrients, and hormones. Cell growth on ECM was quantitated on days 7-15 by [3H]thymidine uptake, cell counting, and total protein. Tumor cells were characterized by flow cytometry and cytology. It was observed that ECM provides superior culture conditions for urological carcinomas. By increasing the initial number of cells plated on ECM and by adding different growth factors or hormones, the growth rate for specific tumor types was increased significantly. Several tumors (11 cases) grown on ECM were examined under the light microscope, and in all cases pre- and post-cytology confirmed malignancy. Tumor cells maintained on ECM and transplanted into nude mide retained their tumorigenic and morphological characteristics. Clinically aggressive tumors were associated with extensive ECM degradation. In addition, the growth of fresh human tumors on ECM provides a biologically relevant model system (for assessing the invasiveness of tumors in vitro) and should also be useful for drug evaluation studies.
Details
- Title: Subtitle
- Growth of human urological tumors on extracellular matrix as a model for the in vitro cultivation of primary human tumor explants
- Creators
- K Pavelic - Grace cancer drug center, Buffalo NY 14263, United StatesM. A Bulbul - Grace cancer drug center, Buffalo NY 14263, United StatesH. K Slocum - GraceZ. P Pavelic - GraceY. M Rustum - Grace cancer drug center, Buffalo NY 14263, United StatesM. J Niedbala - Grace cancer drug center, Buffalo NY 14263, United StatesR. J Bernacki - Grace cancer drug center, Buffalo NY 14263, United States
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.46(7), pp.3653-3662
- PMID
- 3708595
- NLM abbreviation
- Cancer Res
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Publisher
- American Association for Cancer Research
- Language
- English
- Date published
- 1986
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359560602771
Metrics
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