Journal article
Growth stimulation of Porphyromonas endodontalis by hemoglobin and protoporphyrin IX
Oral microbiology and immunology, Vol.15(6), pp.365-370
Accepted for publication February 10, 2000
12/2000
DOI: 10.1034/j.1399-302x.2000.150605.x
PMID: 11154433
Abstract
Porphyromonas endodontalis, like other Porphyromonas species, has a complex set of nutritional requirements. In addition to being an obligate anaerobe, the bacterium must be grown in a complex medium consisting of amino acids, reducing agents and heme compounds. P. endodontalis accumulates high concentrations of heme pigments to the extent that colonies appear black on blood agar. This accumulation of heme and the need for these compounds has been characterized as iron requirements by these species. However, in our studies, P. endodontalis demonstrated growth dependence on hemoglobin or protoporphyrin IX but not on free iron. Iron added to other heme compounds actually decreased growth stimulation by porphyrin-containing compounds. P. endodontalis actively transported free iron, but this process did not appear to be critical for growth. The maximum stimulation of growth by protoporphyrin IX, under conditions of iron deprivation, suggests that P. endodontalis requires the porphyrin moiety as a growth factor.
Details
- Title: Subtitle
- Growth stimulation of Porphyromonas endodontalis by hemoglobin and protoporphyrin IX
- Creators
- M. A. Zerr - Minot Public SchoolsC. D. Cox - UAMS College of MedicineW. T. Johnson - University of IowaD. R. Drake - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Oral microbiology and immunology, Vol.15(6), pp.365-370
- Edition
- Accepted for publication February 10, 2000
- Publisher
- Munksgaard International Publishers
- DOI
- 10.1034/j.1399-302x.2000.150605.x
- PMID
- 11154433
- ISSN
- 0902-0055
- eISSN
- 1399-302X
- Number of pages
- 6
- Language
- English
- Date published
- 12/2000
- Academic Unit
- Microbiology and Immunology; Endodontics; Dental Research
- Record Identifier
- 9984367627302771
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