Journal article
Guanine nucleotides are potent secretagogues in permeabilized parathyroid cells
FEBS letters, Vol.208(1), pp.99-104
1986
DOI: 10.1016/0014-5793(86)81540-X
PMID: 3770214
Abstract
We studied the effects of GTP and its' analogues on PTH release in permeabilized parathyroid cells to assess their role in mediating the unusual inverse relationship between Ca
2+ and PTH release in intact parathyroid cells. Both 10-5 M GppNHp and GTPγS, nonhydrolysable analogues of GTP, produce up to an 8-fold enhancement of PTH release, which is dose-dependent. This effect is specific for GTP analogues as we could not mimic it with other nucleotides. 10
−3 M GDPβS, a nonhydrolysable GDP analogue, completely abolishes GppNHp-stimulated hormone release, providing further support for mediation of this effect by a guanine-nucleotide regulatory protein. In GppNHp-stimulated cells, PTH release is maximal at free [Ca
2+] less than 200 nM and progressively decreases as the free [Ca
2+] increases from 300 nM to 100 μM. These results suggest the presence of a guanine-nucleotide binding protein in the parathyroid cell that may play an important role in the regulation of PTH secretion by Ca
2+ and perhaps other secretagogues.
Details
- Title: Subtitle
- Guanine nucleotides are potent secretagogues in permeabilized parathyroid cells
- Creators
- Marguerite Oetting - Duke UniversityMeryl LeBoff - Brigham and Women's HospitalLinda Swiston - Brigham and Women's HospitalJoseph Preston - Brigham and Women's HospitalEdward Brown - Brigham and Women's Hospital
- Resource Type
- Journal article
- Publication Details
- FEBS letters, Vol.208(1), pp.99-104
- DOI
- 10.1016/0014-5793(86)81540-X
- PMID
- 3770214
- NLM abbreviation
- FEBS Lett
- ISSN
- 0014-5793
- eISSN
- 1873-3468
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1986
- Academic Unit
- Stead Family Department of Pediatrics; General Pediatrics and Adolescent Medicine
- Record Identifier
- 9984354150202771
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