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Guanosine 5'-O-(3-thiotrisphosphate) potentiates both thrombin- and platelet-derived growth factor-induced inositol phosphate release in permeabilized vascular smooth muscle cells. Signaling mechanisms distinguished by sensitivity to pertussis toxin and phorbol esters
Journal article   Open access   Peer reviewed

Guanosine 5'-O-(3-thiotrisphosphate) potentiates both thrombin- and platelet-derived growth factor-induced inositol phosphate release in permeabilized vascular smooth muscle cells. Signaling mechanisms distinguished by sensitivity to pertussis toxin and phorbol esters

Chou-Long Huang and Harlan E Ives
The Journal of biological chemistry, Vol.264(8), pp.4391-4397
01/01/1989
DOI: 10.1016/S0021-9258(18)83754-1
PMID: 2494171
url
https://doi.org/10.1016/S0021-9258(18)83754-1View
Published (Version of record) Open Access

Abstract

The authors compared the mechanisms by which thrombin and platelet-derived growth factor (PDGF) activate phospholipase C in cultured vascular smooth muscle cells. Thrombin caused a transient (< 5 min) increase in inositol trisphosphate (IP sub(3)) while PDGF caused a sustained (> 10 min) increase. Thrombin and PDGF activate phospholipase C by signal transduction systems which differ in kinetic properties and in sensitivity to PMA and pertussis toxin. Despite these differences, both systems appear to involve GTP binding proteins at some step.
guanosine 5-O-(3-thiotrisphosphate) platelet-derived growth factor thrombin

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