Journal article
Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
Microbial cell, Vol.6(1), pp.1-64
01/01/2019
DOI: 10.15698/mic2019.01.664
PMCID: PMC6334234
PMID: 30652105
Abstract
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
Details
- Title: Subtitle
- Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
- Creators
- Hannah L Klein - Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USAGiedrė Bačinskaja - Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, DenmarkJun Che - Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USAAnais Cheblal - Friedrich Miescher Institute for Biomedical Research (FMI), 4058 Basel, SwitzerlandRajula Elango - Department of Biology, University of Iowa, Iowa City, IA, USAAnastasiya Epshtein - Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USADevon M Fitzgerald - Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA. Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA. Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USABelén Gómez-González - Centro Andaluz de BIología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla, Seville, SpainSharik R Khan - Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USASandeep Kumar - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USABryan A Leland - Yale School of Medicine, New Haven, CT, USALéa Marie - Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USAQian Mei - Systems, Synthetic and Physical Biology Graduate Program, Rice University, Houston, TX, USAJudith Miné-Hattab - Institut Curie, PSL Research University, CNRS, UMR3664, F-75005 Paris, France. Sorbonne Université, Institut Curie, CNRS, UMR3664, F-75005 Paris, FranceAlicja Piotrowska - NanoBioMedical Centre, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, PolandErica J Polleys - Department of Biology, Tufts University, Medford, MA USAChristopher D Putnam - Ludwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, CA, USA. Department of Medicine, University of California School of Medicine, San Diego, La Jolla, CA, USAElina A Radchenko - Department of Biology, Tufts University, Medford, MA USAAnissia Ait Saada - Institut Curie, PSL Research University, CNRS, UMR3348 F-91405, Orsay, France. University Paris Sud, Paris-Saclay University, CNRS, UMR3348, F-91405, Orsay, FranceCynthia J Sakofsky - Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, Durham, NC, USAEun Yong Shim - Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USAMathew Stracy - Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UKJun Xia - Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA. Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA. Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USAZhenxin Yan - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USAYi Yin - Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, NC USAAndrés Aguilera - Centro Andaluz de BIología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla, Seville, SpainJuan Lucas Argueso - Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USACatherine H Freudenreich - Department of Biology, Tufts University, Medford, MA USA. Program in Genetics, Tufts University, Boston, MA, USASusan M Gasser - Friedrich Miescher Institute for Biomedical Research (FMI), 4058 Basel, SwitzerlandDmitry A Gordenin - Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, Durham, NC, USAJames E Haber - Department of Biology and Rosenstiel Basic Medical Sciences Research Center Brandeis University, Waltham, MA, USAGrzegorz Ira - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USASue Jinks-Robertson - Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC USAMegan C King - Yale School of Medicine, New Haven, CT, USARichard D Kolodner - Ludwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, CA, USA. Department of Cellular and Molecular Medicine, University of California School of Medicine, San Diego, La Jolla, CA, USA. Moores-UCSD Cancer Center, University of California School of Medicine, San Diego, La Jolla, CA, USA. Institute of Genomic Medicine, University of California School of Medicine, San Diego, La Jolla, CA, USAAndrei Kuzminov - Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USASarah AE Lambert - Institut Curie, PSL Research University, CNRS, UMR3348 F-91405, Orsay, France. University Paris Sud, Paris-Saclay University, CNRS, UMR3348, F-91405, Orsay, FranceSang Eun Lee - Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USAKyle M Miller - Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA. Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USASergei M Mirkin - Department of Biology, Tufts University, Medford, MA USAThomas D Petes - Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, NC USASusan M Rosenberg - Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA. Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA. Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA. Systems, Synthetic and Physical Biology Graduate Program, Rice University, Houston, TX, USARodney Rothstein - Department of Genetics & Development, Columbia University Irving Medical Center, New York, NY, USALorraine S Symington - Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USAPawel Zawadzki - NanoBioMedical Centre, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, PolandNayun Kim - Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston, Houston, TX, USAMichael Lisby - Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, DenmarkAnna Malkova - Department of Biology, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Microbial cell, Vol.6(1), pp.1-64
- DOI
- 10.15698/mic2019.01.664
- PMID
- 30652105
- PMCID
- PMC6334234
- NLM abbreviation
- Microb Cell
- ISSN
- 2311-2638
- eISSN
- 2311-2638
- Publisher
- Shared Science Publishers OG
- Alternative title
- Cellular assays for DNA repair and recombination
- Language
- English
- Date published
- 01/01/2019
- Academic Unit
- Biology
- Record Identifier
- 9984217542502771
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