Journal article
Gα13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation
Development (Cambridge), Vol.151(19), dev202597
09/30/2024
DOI: 10.1242/dev.202597
PMCID: PMC11463957
PMID: 39258889
Appears in UI Libraries Support Open Access
Abstract
Pharyngeal endoderm cells undergo convergence and extension (C&E), which is essential for endoderm pouch formation and craniofacial development. Our prior work implicates Gα13/RhoA-mediated signaling in regulating this process, but underlying mechanisms remain unclear. Here, we used endoderm-specific transgenic and Gα13 mutant zebrafish to demonstrate that Gα13 plays a crucial role in pharyngeal endoderm C&E by regulating RhoA activation and E-cadherin expression. We showed that during C&E, endodermal cells gradually establish stable cell-cell contacts, acquire apical-basal polarity, and undergo actomyosin-driven apical constriction, processes that require Gα13. Additionally, we found Gα13-deficient embryos exhibit reduced E-cadherin expression, partially contributing to endoderm C&E defects. Notably, interfering with RhoA function disrupts spatial actomyosin activation without affecting E-cadherin expression. Collectively, our findings identify critical cellular processes for pharyngeal endoderm C&E and reveal that Gα13 controls this through two independent pathways: modulating RhoA activation and regulating E-cadherin expression, thus unveiling intricate mechanisms governing pharyngeal endoderm morphogenesis.
Details
- Title: Subtitle
- Gα13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation
- Creators
- Bo Hu - University of Iowa, Anatomy and Cell BiologyJoshua Pinzour - University of IowaAsmi Patel - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USAFaith Rooney - University of IowaAmie Zerwic - University of IowaYuanyuan Gao - University of Iowa, Anatomy and Cell BiologyNhan T Nguyen - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USAHuaping Xie - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USADing Ye - University of IowaFang Lin - University of Iowa, Anatomy and Cell Biology
- Resource Type
- Journal article
- Publication Details
- Development (Cambridge), Vol.151(19), dev202597
- DOI
- 10.1242/dev.202597
- PMID
- 39258889
- PMCID
- PMC11463957
- NLM abbreviation
- Development
- ISSN
- 0950-1991
- eISSN
- 1477-9129
- Publisher
- The Company of Biologists
- Grant note
- IOS-1354457 / National Science Foundation
- Language
- English
- Date published
- 09/30/2024
- Academic Unit
- Anatomy and Cell Biology; Stead Family Department of Pediatrics; Craniofacial Anomalies Research Center; Otolaryngology; Internal Medicine
- Record Identifier
- 9984720725402771
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