Journal article
H2O2-induced O2⋅‒ Production by a Non-phagocytic NAD(P)H Oxidase Causes Oxidant Injury
The Journal of biological chemistry, Vol.276(31), pp.29251-29256
05/17/2001
DOI: 10.1074/jbc.M102124200
PMCID: PMC3974124
PMID: 11358965
Abstract
Non-phagocytic NAD(P)H oxidases have been implicated as major sources of reactive oxygen species in blood vessels. These oxidases can be activated by cytokines, thereby generating
O
2
⋅
‒
, which is subsequently converted to H
2
O
2
and other oxidant species. The oxidants, in turn, act as important second messengers in cell signaling cascades. We hypothesized that reactive oxygen species, themselves, can activate the non-phagocytic NAD(P)H oxidases in vascular cells to induce oxidant production and, consequently, cellular injury. The current report demonstrates that exogenous exposure of non-phagocytic cell types of vascular origin (smooth muscle cells and fibroblasts) to H
2
O
2
activates these cell types to produce
O
2
⋅
‒
via an NAD(P)H oxidase. The ensuing endogenous production of
O
2
⋅
‒
contributes significantly to vascular cell injury following exposure to H
2
O
2
. These results suggest the existence of a feed-forward mechanism, whereby reactive oxygen species such as H
2
O
2
can activate NAD(P)H oxidases in non-phagocytic cells to produce additional oxidant species, thereby amplifying the vascular injury process. Moreover, these findings implicate the non-phagocytic NAD(P)H oxidase as a novel therapeutic target for the amelioration of the biological effects of chronic oxidant stress.
Details
- Title: Subtitle
- H2O2-induced O2⋅‒ Production by a Non-phagocytic NAD(P)H Oxidase Causes Oxidant Injury
- Creators
- Wei-Gen Li - University of IowaFrancis J. Miller - University of IowaHannah J. Zhang - University of IowaDouglas R. Spitz - University of IowaLarry W. Oberley - University of IowaNeal L. Weintraub - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.276(31), pp.29251-29256
- DOI
- 10.1074/jbc.M102124200
- PMID
- 11358965
- PMCID
- PMC3974124
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 05/17/2001
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984312985302771
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