Journal article
HBD3 Induces PD-L1 Expression on Head and Neck Squamous Cell Carcinoma Cell Lines
Antibiotics (Basel), Vol.8(4), p.161
09/24/2019
DOI: 10.3390/antibiotics8040161
PMCID: PMC6963492
PMID: 31554151
Abstract
Human β-defensin 3 (HBD3) is an antimicrobial peptide up-regulated in the oral tissues of individuals with head and neck squamous cell carcinomas (HNSCC) and oral squamous cell carcinomas (SCC) and present in high concentrations in their saliva. In this study, we determined if HBD3 contributes to HNSCC pathogenesis by inducing programmed death-ligand 1 (PD-L1) expression on HNSCC cell lines. For this, SCC cell lines SCC4, SCC15, SCC19, SCC25, and SCC99 (5.0 × 10
4
viable cells) were used. Cells were incubated with IFNγ (0.6 µM) and HBD3 (0.2, 2.0, or 20.0 µM) for 24 h. Cells alone served as controls. Cells were then treated with anti-human APC-CD274 (PD-L1) and Live/Dead Fixable Green Dead Cell Stain. Cells treated with an isotype antibody and cells alone served as controls. All cell suspensions were analyzed in a LSR II Violet Flow Cytometer. Cytometric data was analyzed using FlowJo software. Treatment with IFNγ (0.6 µM) increased the number of cells expressing PD-L1 (
p
< 0.05) with respect to controls. Treatment with HBD3 (20.0 µM) also increased the number of cells expressing PD-L1 (
p
< 0.05) with respect to controls. However, treatment with IFNγ (0.6 µM) was not significantly different from treatment with HBD3 (20.0 µM) and the numbers of cells expressing PD-L1 were similar (
p
= 1). Thus, HBD3 increases the number of cells expressing PD-L1. This is a novel concept, but the role HBD3 contributes to HNSCC pathogenesis by inducing PD-L1 expression in tumors will have to be determined.
Details
- Title: Subtitle
- HBD3 Induces PD-L1 Expression on Head and Neck Squamous Cell Carcinoma Cell Lines
- Creators
- Maria Paula Gomez HernandezAmber M. Bates - University of Wisconsin–MadisonEmily E. Starman - University of IowaEmily A. Lanzel - University of IowaCarissa Comnick - University of IowaXian Jin Xie - University of IowaKim A. Brogden - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Antibiotics (Basel), Vol.8(4), p.161
- DOI
- 10.3390/antibiotics8040161
- PMID
- 31554151
- PMCID
- PMC6963492
- NLM abbreviation
- Antibiotics (Basel)
- ISSN
- 2079-6382
- eISSN
- 2079-6382
- Publisher
- MDPI
- Grant note
- DOI: 10.13039/100000009, name: National Institutes of Health, award: R01 DE014390, T90 DE023520
- Language
- English
- Date published
- 09/24/2019
- Academic Unit
- Preventive and Community Dentistry; Oral Pathology, Radiology and Medicine; Biostatistics; Dental Research; Periodontics
- Record Identifier
- 9984367719802771
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