Journal article
HER-2/neu gene amplification characterized by fluorescence in situ hybridization : Poor prognosis in node-negative breast carcinomas
Journal of clinical oncology, Vol.15(8), pp.2894-2904
1997
DOI: 10.1200/JCO.1997.15.8.2894
PMID: 9256133
Abstract
PURPOSE
The HER-2/neu gene codes for a membrane receptor protein that is homologous, but distinct from the epidermal growth factor receptor. This investigation was performed to validate fluorescence in situ hybridization (FISH) as a sensitive and specific method for assessing HER-2/neu gene amplification in archival tissue and to test whether this alteration is associated with poor prognosis.
MATERIALS AND METHODS
HER-2/neu gene amplification was determined by FISH in 140 archival breast cancers, previously characterized for gene amplification by Southern hybridization or dot-blot hybridization, and for gene expression by Northern hybridization, Western immunoblot, or immunohistochemistry. A separate cohort of 324 node-negative breast cancers was assessed for amplification by FISH to determine the utility of HER-2/neu gene amplification.
RESULTS
Relative to solid-matrix blotting procedures, FISH analysis of HER-2/neu gene amplification showed a sensitivity of 98% and a specificity of 100% in 140 breast cancers. Among patients treated by surgery only, the relative risks (relative hazard) of early recurrence (recurrent disease within 24 months of diagnosis), recurrent disease (at any time), and disease-related death were statistically significantly associated with amplification. The prognostic information contributed by HER-2/neu amplification was independent of the other markers studied.
CONCLUSION
FISH was an alternative technique for determining gene amplification and had some distinct advantages over Southern hybridization. Our results demonstrate that HER-2/neu gene amplification in the absence of adjuvant therapy is an independent predictor of poor clinical outcome and is a stronger discriminant than tumor size. Women with small tumors that had gene amplification were at increased risk of recurrence and disease-related death.
Details
- Title: Subtitle
- HER-2/neu gene amplification characterized by fluorescence in situ hybridization : Poor prognosis in node-negative breast carcinomas
- Creators
- Michael F Press - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesLeslie Bernstein - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesP. A THOMAS - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesLorraine F Meisner - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesJian-Yuan Zhou - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesYanling Ma - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesG HUNG - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesR. A ROBINSON - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesCharles Harris - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesA EL-NAGGAR - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesD. J SLAMON - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesRichard N Phillips - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesJ. S ROSS - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesS. R WOLMAN - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United StatesK. J FLOM - Cytogenetics Section, State Laboratory of Hygiene, University of Wisconsin, Madison, WI, United States
- Resource Type
- Journal article
- Publication Details
- Journal of clinical oncology, Vol.15(8), pp.2894-2904
- Publisher
- Lippincott Williams & Wilkins; Baltimore, MD
- DOI
- 10.1200/JCO.1997.15.8.2894
- PMID
- 9256133
- ISSN
- 0732-183X
- eISSN
- 1527-7755
- Language
- English
- Date published
- 1997
- Academic Unit
- Oral Pathology, Radiology and Medicine; Pathology
- Record Identifier
- 9984047671302771
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