Journal article
HIV-1 ICD p24 antigen detection in Ugandan infants: Use in early diagnosis of infection and as a marker of disease progression
Journal of medical virology, Vol.62(4), pp.426-434
2000
DOI: 10.1002/1096-9071(200012)62:4<426::AID-JMV6>3.0.CO;2-S
PMID: 11074470
Abstract
The objective of this study was to determine the use of immune-complex dissociated (ICD) p24 antigen detection for the diagnosis and prognosis of HIV-1 infection in Ugandan children. Plasma collected prospectively from children born to HIV-1 infected Ugandan women was stored and later analyzed for the presence of neutralizable HIV-1 p24 antigen using the Coulter ICD p24 antigen and neutralization kits. HIV-1 infection status, disease progression, and survival of the children were determined. Specimens from 311 children born to HIV-1 infected women, including 138 HIV-1 infected children, and 113 children born to negative women were tested. Sixty-nine (50%) infected children were p24 antigen positive at least once. For early HIV-1 diagnosis, the specificity and positive predictive value of the assay were consistently high (>95% and >83% respectively), but the sensitivity was low (6-53%), especially in the first months of life. The presence of p24 antigenemia in the first two years of life was associated with poor survival (20%) by 80 months of age compared with infected children without antigenemia (43%, P < 0.001). Early detection of p24 antigen (</=2 months) was associated with higher mortality than first detection at an older age (>6 months, P < 0.001). The data suggest that ICD p24 antigen detection is not a sensitive method for the determination of infant HIV-1 status in our cohort of HIV-1 infected Ugandan children tested in the first two years of life. There was a strong correlation, however, between the presence and time of onset of p24 antigenemia and mortality among HIV-1 infected children.
Details
- Title: Subtitle
- HIV-1 ICD p24 antigen detection in Ugandan infants: Use in early diagnosis of infection and as a marker of disease progression
- Creators
- Laura A GUAY - Department of Pathology, The Johns Hopkins University, Baltimore, Maryland, United StatesDavid L HOM - Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, United StatesSam R KABENGERA - Ministry of Health, Entebbe, UgandaEstelle M PIWOWAR-MANNING - Department of Pathology, The Johns Hopkins University, Baltimore, Maryland, United StatesPeter KATAAHA - Ministry of Health, Entebbe, UgandaChristopher NDUGWA - Department of Paediatrics, Makerere University, Kampala, UgandaLawrence H MARUM - Department of Pathology, The Johns Hopkins University, Baltimore, Maryland, United StatesIsrael KALYESUBULA - Department of Paediatrics, Makerere University, Kampala, UgandaJ. Brooks JACKSON - Department of Pathology, The Johns Hopkins University, Baltimore, Maryland, United States
- Resource Type
- Journal article
- Publication Details
- Journal of medical virology, Vol.62(4), pp.426-434
- Publisher
- Wiley-Liss; New York, NY
- DOI
- 10.1002/1096-9071(200012)62:4<426::AID-JMV6>3.0.CO;2-S
- PMID
- 11074470
- ISSN
- 0146-6615
- eISSN
- 1096-9071
- Language
- English
- Date published
- 2000
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984047983102771
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