HIV-1 Infection Induces Acetylation of NPM1 That Facilitates Tat Localization and Enhances Viral Transactivation

Shrikanth S. Gadad; Roshan Elizabeth Rajan; Parijat Senapati; Snehajyoti Chatterjee; Jayasha Shandilya; Prasanta Kumar Dash; Udaykumar Ranga; Tapas K. Kundu

doi:10.1016/j.jmb.2011.04.009

Back

HIV-1 Infection Induces Acetylation of NPM1 That Facilitates Tat Localization and Enhances Viral Transactivation

Journal article

Peer reviewed

HIV-1 Infection Induces Acetylation of NPM1 That Facilitates Tat Localization and Enhances Viral Transactivation

Shrikanth S. Gadad, Roshan Elizabeth Rajan, Parijat Senapati, Snehajyoti Chatterjee, Jayasha Shandilya, Prasanta Kumar Dash, Udaykumar Ranga and Tapas K. Kundu

Journal of molecular biology, Vol.410(5), pp.997-1007

07/29/2011

DOI: 10.1016/j.jmb.2011.04.009

PMID: 21763502

View Online

Abstract

Human immunodeficiency virus type 1 (HIV-1) following integration hijacks host cell machineries where chromatinization of the viral genome regulates its latency, transcription, and replication. The cooperation among ATP-dependent chromatin remodeling factors, posttranslational modifying enzymes, and histone chaperones is well established during transcriptional activation in eukaryotes. However, the role of histone chaperones in transcription of the HIV promoter is poorly understood. Previous studies from our group have established the role of the human histone chaperone nucleophosmin (NPM1) in the acetylation-dependent chromatin transcription. NPM1 is known to interact with HIV-Tat. Here, we report that infection by HIV-1 induces the acetylation of histone chaperone NPM1. Acetylation of NPM1 was found to be critical for nuclear localization of Tat as well as Tat-mediated transcription alluding to the critical role for the host factor towards viral pathogenesis. Furthermore, knockdown experiments mediated by small interfering RNA identified the critical role played by the chaperone NPM1 in transcriptional activation of the integrated provirus. These results shed further insights into the possible role of histone chaperone NPM1 acetylation in viral gene transcription, which could be a potential therapeutic target. (C) 2011 Elsevier Ltd. All rights reserved.

Biochemistry & Molecular Biology

Life Sciences & Biomedicine

Science & Technology

Details

Title: Subtitle: HIV-1 Infection Induces Acetylation of NPM1 That Facilitates Tat Localization and Enhances Viral Transactivation
Creators: Shrikanth S. Gadad - Jawaharlal Nehru Centre for Advanced Scientific Research
Roshan Elizabeth Rajan - Jawaharlal Nehru Centre for Advanced Scientific Research
Parijat Senapati - Jawaharlal Nehru Centre for Advanced Scientific Research
Snehajyoti Chatterjee - Jawaharlal Nehru Centre for Advanced Scientific Research
Jayasha Shandilya - Jawaharlal Nehru Centre for Advanced Scientific Research
Prasanta Kumar Dash - Jawaharlal Nehru Centre for Advanced Scientific Research
Udaykumar Ranga - Jawaharlal Nehru Centre for Advanced Scientific Research
Tapas K. Kundu - Jawaharlal Nehru Centre for Advanced Scientific Research
Resource Type: Journal article
Publication Details: Journal of molecular biology, Vol.410(5), pp.997-1007
DOI: 10.1016/j.jmb.2011.04.009
PMID: 21763502
NLM abbreviation: J Mol Biol
ISSN: 0022-2836
eISSN: 1089-8638
Publisher: Elsevier
Number of pages: 11
Grant note: Department of Biotechnology, Government of India; Department of Biotechnology (DBT) India Jawaharlal Nehru Centre
Language: English
Date published: 07/29/2011
Academic Unit: Iowa Neuroscience Institute; Neuroscience and Pharmacology
Record Identifier: 9984303749302771

Metrics

6 Record Views

27 Times Cited - Web of Science