HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation

Corine St Gelais; Christopher M Coleman; Jian-Hua Wang; Li Wu

doi:10.1371/journal.pone.0034521

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HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation

Journal article

Open access

Peer reviewed

HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation

Corine St Gelais, Christopher M Coleman, Jian-Hua Wang and Li Wu

PloS one, Vol.7(3), pp.e34521-e34521

2012

DOI: 10.1371/journal.pone.0034521

PMCID: PMC3316695

PMID: 22479639

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Abstract

HIV-1 Nef enhances dendritic cell (DC)-mediated viral transmission to CD4(+) T cells, but the underlying mechanism is not fully understood. It is also unknown whether HIV-1 infected DCs play a role in activating CD4(+) T cells and enhancing DC-mediated viral transmission. Here we investigated the role of HIV-1 Nef in DC-mediated viral transmission and HIV-1 infection of primary CD4(+) T cells using wild-type HIV-1 and Nef-mutated viruses. We show that HIV-1 Nef facilitated DC-mediated viral transmission to activated CD4(+) T cells. HIV-1 expressing wild-type Nef enhanced the activation and proliferation of primary resting CD4(+) T cells. However, when co-cultured with HIV-1-infected autologous DCs, there was no significant trend for infection- or Nef-dependent proliferation of resting CD4(+) T cells. Our results suggest an important role of Nef in DC-mediated transmission of HIV-1 to activated CD4(+) T cells and in the activation and proliferation of resting CD4(+) T cells, which likely contribute to viral pathogenesis.

Lymphocyte Activation

Dendritic Cells - immunology

HIV Infections - virology

Humans

Cells, Cultured

T-Lymphocytes - virology

CD4-Positive T-Lymphocytes - immunology

HIV Infections - immunology

HIV-1 - immunology

nef Gene Products, Human Immunodeficiency Virus - immunology

T-Lymphocytes - immunology

CD4-Positive T-Lymphocytes - virology

Dendritic Cells - virology

Details

Title: Subtitle: HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation
Creators: Corine St Gelais - Center for Retrovirus Research, Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America
Christopher M Coleman
Jian-Hua Wang
Li Wu
Resource Type: Journal article
Publication Details: PloS one, Vol.7(3), pp.e34521-e34521
DOI: 10.1371/journal.pone.0034521
PMID: 22479639
PMCID: PMC3316695
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: R21 AI102822 / NIAID NIH HHS R21AI098524 / NIAID NIH HHS R21 AI098524 / NIAID NIH HHS R21 AI078762 / NIAID NIH HHS R01 AI068493 / NIAID NIH HHS AI068493 / NIAID NIH HHS AI078762 / NIAID NIH HHS
Language: English
Date published: 2012
Academic Unit: Microbiology and Immunology
Record Identifier: 9984002375602771

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